沼泽红假单胞菌CGA009砷调控蛋白(ArsR)的调控作用  

Regulatory Role of Arsenic Regulatory Protein(ArsR)in Rhodopseudomonas palustris CGA009

作　　者：金春英[1] 崔亮 陈勇男 杨素萍[1] 赵春贵[1] JIN Chunying;CUI Liang;CHEN Yongnan;YANG Suping;ZHAO Chungui(College of Chemical Engineering,Huaqiao University,Xiamen 361021,China;International College,Beijing University of Agricultural,Beijing 102206,China)

机构地区：[1]华侨大学化工学院,福建厦门361021 [2]北京农学院国际学院,北京102206

出　　处：《华侨大学学报（自然科学版）》2024年第5期618-625,共8页Journal of Huaqiao University(Natural Science)

基　　金：国家海洋公益性行业科研专项(201505026);福建省自然科学基金资助项目(2018J01049)。

摘　　要：采用改良的凝胶电泳迁移滞缓(EMSA)法和砷生物传感法研究沼泽红假单胞菌CGA009菌株6个高度异质性ArsR相关蛋白的调控作用。研究结果表明:6个ArsR相关蛋白都具有As(Ⅲ)依赖的调控活性,改良的EMSA图谱可用于系统评价ArsR的功能;6个相关蛋白对异种(E.coli pR733 or Stapylococcus aureus pⅠ258)的砷抗性操纵子的调控具有不同的选择性,氨基酸序列同源性(甚至低于30%同源性)的ArsR具有砷依赖的调控作用。Using a modified gel electrophoretic mobility shift assay(EMSA)method and Arsenic biosensing method,the regulation of six highly heterogeneous ArsR-associated proteins from Rhodopseudomonas palustris CGA009 bacterial strain is investigated.The reseach results show that six ArsR-associated proteins exhibited As(Ⅲ)-dependent regulatory activity.The modified EMSA mappings can systematically evaluate the functions of ArsR.Six ArsR-associated proteins have different selectivity in regulating the arsenic resistance operon of different species(E.coli pR733 or Staphylococcus aureus pⅠ258).ArsRs with amino acid sequence homology(even with below 30%homology)have arsenic-dependent regulation.

关 键 词：沼泽红假单胞菌 砷 砷调控蛋白 砷生物传感 

分 类 号：Q811.4[生物学—生物工程]