产灵菌红素沙雷氏菌R18的鉴定及基因组特性  

Identification and Genomic Characterization of Prodigiosin-Producing Serratia sp.R18

作　　者：陈明霞[1] 邢元军 李和阳 施俊豪 CHEN Mingxia;XING Yuanjun;LI Heyang;SHI Junhao(College of Chemical Engineering,Huaqiao University,Xiamen 361021,China;Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration,Third Institute of Oceanography,Xiamen 361005,China)

机构地区：[1]华侨大学化工学院,福建厦门361021 [2]自然资源部第三海洋研究所,福建厦门361005 [3]第三海洋研究所福建省海洋生态保护与修复重点实验室,福建厦门361005

出　　处：《华侨大学学报（自然科学版）》2024年第5期626-634,共9页Journal of Huaqiao University(Natural Science)

基　　金：福建省海洋经济发展专项资金资助项目(FJHJF-L-2022-11);华侨大学2023年实验教学与管理改革课题(SY2023J05);福建省海洋生态保护与修复重点实验室开放基金资助项目(EPR2020002)。

摘　　要：采用形态学、生理生化和分子生物学方法对菌株R18进行菌种鉴定。通过基因组测序和生物信息学比较,进一步分析其分类地位和生物学特性,并在基因组水平上探讨该菌株红色素的合成基因簇和代谢路径。结果表明:菌株R18最佳生长条件为温度30~37℃,pH值6~8,NaCl质量浓度0~10 g·L^(-1);菌株R18与粘质沙雷氏菌模式菌株ATCC 13880的16S rDNA相似度为99.86%,基因组平均核苷酸均一性和数字DNA-DNA杂交值分别为98.73%,89.5%,均高于物种界定阈值,属于同一个物种;菌株R18灵菌红素合成基因簇全长35021 bp,包含29个基因,其中,4个核心合成基因、10个补充的合成基因、1个调控基因、1个转运基因及13个其他基因,具有完整的灵菌红素合成代谢途径。Strain R18 was identified using morphological,physiological,biochemical and molecular biological methods.Its taxonomic status and biological properties were further analyzed by comparing genome sequencing and bioinformatics,and the synthetic gene cluster and metabolic pathway of the red pigment in this strain were explored at the genomic level.The results showed that the optimal growth conditions for strain R18 were temperature of 30-37℃,pH value of 6-8,and the mass concentration of NaCl of 0-10 g·L^(-1).Strain R18 shared a 16S rDNA similarity of 99.86%to Serratia marcescens type strain ATCC 13880,the genome average nucleotide identity value and the digital DNA-DNA hybridization value were 98.73%and 89.5%,respectively,both of which were higher than the species identification threshold and belong to the same specie.Strain R18 prodigiosin synthetic gene cluster had a total length of 35021 bp and contained 29 genes including 4 core syn thetic genes,10 complementary synthetic genes,1 regulatory gene,1 transporter gene and 13 other genes,it had a complete prodigiosin synthesis metabolic pathway.

关 键 词：粘质沙雷氏菌 灵菌红素 菌种鉴定 基因组特性 

分 类 号：Q939.1[生物学—微生物学]