包封骨形态发生蛋白2的不同相对分子质量聚乳酸-聚乙醇酸共聚物微囊促进成骨效果的研究  

Osteogenic effect of poly(lactic-co-glycolic acid) microcapsules with different molecular weights encapsulating bone morphogenetic protein 2

作　　者：袁莉红 陈晨[1,2,3] 马语笛 梁睿贞 Yuan Lihong;Chen Chen;Ma Yudi;Liang Ruizhen(Dept.of Endodontics,the Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China;State Key Laboratory Cultivation Base of Research,Prevention and Treatment for Oral Diseases,Nanjing 210029,China;Jiangsu Province Engineering Research Center of Stomatological Translational Medicine,Nanjing 210029,China;The Seventh Clinic,the Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院牙体牙髓科,南京210029 [2]口腔疾病研究与防治国家级重点实验室培育建设点,南京210029 [3]江苏省口腔转化医学工程研究中心,南京210029 [4]南京医科大学附属口腔医院第七门诊部,南京210029

出　　处：《华西口腔医学杂志》2024年第5期572-580,共9页West China Journal of Stomatology

基　　金：国家自然科学基金(81970927);江苏省重点研发计划社会发展项目(BE2022797)。

摘　　要：目的 探索不同相对分子质量聚乳酸-聚乙醇酸共聚物(PLGA)微囊包封骨形态发生蛋白2 (BMP-2)对成骨细胞骨形成能力的影响。方法 用双通道微量注射泵制备包封BMP-2的2种相对分子质量(12 000和30 000)的PLGA微囊。用光学显微镜及扫描电子显微镜(SEM)观察微囊形态结构;磷酸盐缓冲溶液浸泡法表征微囊缓释性能;细胞Calcein-AM/PI染色及CCK-8法检测微囊细胞相容性;Transwell迁移实验检测包封BMP-2的微囊作用48 h对MC3T3-E1细胞的趋化作用;碱性磷酸酶活力测定、茜素红染色法检测微囊作用MC3T3-E1细胞后对细胞骨形成能力的影响。结果 2种相对分子质量的微囊均表面光滑,具有良好的细胞相容性。相对分子质量12 000的微囊的趋化作用最佳。相对分子质量30 000的微囊较相对分子质量12 000的微囊缓释时间长,且初始爆发量减少了约25%。成骨诱导14、21 d后,相对分子质量30 000的微囊形成的钙沉积结节较相对分子质量12 000的微囊多。结论 本研究通过调控PLGA的相对分子质量控制BMP-2的释放,发现相对分子质量30 000的微囊能够更好地诱导MC3T3-E1细胞的长期骨形成能力。Objective This study aimed to explore the effects of bone morphogenetic protein 2(BMP-2) encapsulated in poly(lactic-co-glycolic acid)(PLGA) microcapsules with different molecular weights on the osteogenic ability of osteoblasts.Methods PLGA microcapsules with different molecular weights(12 000,30 000) encapsulating BMP-2,were prepared using a dual-channel microinjection pump.The morphology and structure of the microcapsules were characterized by optical microscopy and scanning electron microscopy.The sustained-release performance of the microcapsules was characterized by phosphate buffered saline immersion method.The cell compatibility of the microcapsules was detected by the Calcein-AM/PI staining and CCK-8 method.The chemotactic effect of BMP-2-encapsulated microcapsules on MC3T3-E1 cells after 48 h of treatment was detected by the Transwell assay.The alkaline phosphatase activity assay and Alizarin Red S staining were used to characterize the effect of microcapsules on the osteogenic ability of MC3T3-E1 cells.Results Both types of microcapsules with different molecular weights exhibited smooth surfaces,as well as uniform and good cell compatibility.The chemotactic effect of the 12 000 microcapsules was outstanding.The 30 000 microcapsules had a longer sustainedrelease time,and the initial burst release was reduced by approximately 25% compared with the 12 000 microcapsules.In addition,30 000 microcapsules performed better in long-term osteogenesis induction than 12 000 microcapsules.Conclusion In this study,the release of BMP-2 is regulated by adjusting the molecular weight of PLGA,and the results indicate that 30 000 microcapsules can better induce the long-term osteogenic ability of MC3T3-E1 cells.

关 键 词：聚乳酸-聚乙醇酸共聚物微囊 骨形态发生蛋白2 成骨 

分 类 号：R318.08[医药卫生—生物医学工程]