基于靶向代谢组学技术对新疆高原肺动脉高压人群特征的初步研究  

A preliminary study on characteristics of high altitude pulmonary hypertension in the Xinjiang population based on targeted metabolomics technology

作　　者：杨小玲 艾尼外尔·吐尔逊 迪丽努尔·买买提依明[1] YANG Xiaoling;Ainiwaier Turxun;Dilinur Maimaitiyiming(Department of General Cardiology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of Internal Medicine,Uyghur Medicine Hospital of Akto County,Akto Xinjiang 845550,China)

机构地区：[1]新疆医科大学第一附属医院综合心脏内科,乌鲁木齐830054 [2]新疆阿克陶县维吾尔医医院内科,新疆阿克陶845550

出　　处：《新疆医科大学学报》2024年第9期1207-1214,共8页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区“天山英才”医药卫生高层次人才培养计划项目(TSYC202301A020)。

摘　　要：目的通过代谢组学技术筛选出与低氧肺动脉高压(Hypoxic pulmonary hypertension,HPH)相关的潜在生物标志物和代谢通路。方法采用液相色谱-串联质谱联用技术(LCMS/MS)对新疆帕米尔高原20例HPH患者及14例健康对照者的血清样本进行分析。结果共鉴定出325种代谢物,其中78种存在显著差异。这些差异代谢物包括5-羟基色胺、尿囊素和二甲基鸟苷等,它们可能作为潜在的生物标志物。并通过京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析,筛选出4条与HPH发病机制相关的关键代谢通路,包括丁酸代谢(Butanoate metabolism)、咖啡因代谢(Caffeine metabolism)、胰高血糖素信号通路(Glucagon signaling pathway)和柠檬酸循环(Citrate cycle/TCA cycle)。这些通路主要涉及生物合成、能量代谢、氧化应激和炎症,可能在HPH的发生和发展中发挥重要作用。结论本研究揭示了HPH的代谢特征,发现了一系列潜在的生物标志物和代谢通路,这些发现为进一步研究HPH的发病机制提供了重要线索,并为HPH的诊断和治疗提供了新的靶点和思路.Objective To identify potential biomarkers and metabolic pathways associated with hypoxic pulmonary hypertension(HPH)using metabolomics technology.Methods Serum samples from 20 HPH patients and 14 healthy controls from the Pamir Plateau in Xinjiang were analyzed using liquid chromatography-tandem mass spectrometry(LC-MS/MS).Results A total of 325 metabolites were identified,of which 78 showed significant differences.These differential metabolites include 5-hydroxytryptamine,allantoin and dimethylguanosine,which may serve as potential biomarkers.Kyoto encyclopedia of genes and genomes(KEGG)analysis identified four key metabolic pathways related to the pathogenesis of HPH:Butanoate metabolism,Caffeine metabolism,Glucagon signaling pathway and Citrate cycle(TCA cycle).These pathways were primarily involved in biosynthesis,energy metabolism,oxidative stress and inflammation,potentially playing important roles in the onset and progression of HPH.Conclusion This study reveals the metabolic characteristics of HPH,identifying a series of potential biomarkers and metabolic pathways.These findings provide important insights for further research into the pathogenesis of HPH and offer new targets and approaches for its diagnosis and treatment.

关 键 词：低氧肺动脉高压 代谢组学 生物标志物 通路 

分 类 号：R563[医药卫生—呼吸系统]