维吾尔族习用药神香草特征图谱与含量测定  

Establishment of specific chromatogram of Uyghur traditional medicine Herba Hyssopi and determination of two constituents

作　　者：苏建 朱靖 孔亚萍 尹璇 赵振霞 刘永利 SU Jian;ZHU Jing;KONG Yaping;YIN Xuan;ZHAO Zhenxia;LIU Yongli(NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine,Laboratory for Inspection of Traditional Chinese Medicine,Hebei Institute for Drug and Medical Device Control,Shijiazhuang 052000,China;Zhangzhou Drug Inspection Institute,Zhangzhou 363000,China;The Pharmacovigilance Center of Hebei,Shijiazhuang 050090,China)

机构地区：[1]河北省药品医疗器械检验研究院中药检验室,国家药品监督管理局中药材质量监测评价重点实验室,河北石家庄052000 [2]福建省漳州市药品检验所,福建漳州363000 [3]河北省药物警戒中心,河北石家庄050090

出　　处：《药学研究》2024年第9期862-866,共5页Journal of Pharmaceutical Research

基　　金：河北省高层次人才资助项目(No.C20221109)。

摘　　要：目的建立神香草高效液相色谱(HPLC)特征图谱,地奥司明、蒙花苷的含量测定方法。方法分析采用C18色谱柱(4.6 mm×250 mm,5μm);乙腈-0.1%磷酸为流动相,梯度洗脱;流速1.0 mL·min^(-1);特征图谱检测波长328 nm,含量测定检测波长333 nm。结果神香草HPLC特征图谱中有18个主要色谱峰。地奥司明在10.773~430.92 ng、蒙花苷在0.3247~324.73 ng范围内线性关系良好,平均回收率(n=9)分别为97.1%(RSD=2.2%)、100.2%(RSD=0.7%)。结论该方法准确可靠,可用于神香草的质量控制。Objective To establish the HPLC specific chromatogram of Herba Hyssopi and to determine the contents of diosmin and linarin.Methods The analysis was performed on C 18 column(4.6 mm×250 mm,5μm),mobile phase was acetonitrile-0.1%phosphoric acid solution in a gradient elution manner at a flow rate of 1.0 mL·min^(-1),and detection wavelengths were set at 328 nm for specific chromatogram,and 333 nm for diosmin and linarin.Results There were eighteen main chromatographic peaks in the HPLC specific chromatogram of Hyssopus Officinalis.Diosmin and linarin showed good linear relationships within the ranges of 10.773~430.92 ng and 0.3247~324.73 ng,whose average recoveries(n=9)were 97.1%and 100.2%with the RSD of 1.5%and 0.7%,respectively.Conclusion This method was accurate and stable,which can be used for the quality control of Hyssopus Officinalis.

关 键 词：高效液相色谱 维药 神香草 地奥司明 蒙花苷 特征图谱 相对保留时间 

分 类 号：R927.2[医药卫生—药学]