荧光标记技术在药物成分示踪研究中的应用  

Application of fluorescence labeling technique in drug component tracing

作　　者：董丽敏 杜海涛 王平[2] 胡亚楠 谢仲伯 张会敏[2] 刘善新[2] 师彬 DONG Limin;DU Haitao;WANG Ping;HU Yanan;XIE Zhongbai;ZHANG Huimin;LIU Shanxin;SHI Bin(School of Pharmaceutical Sciences,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Academy of Chinese Medicine,Jinan 250014,China;Shandong Hongjitang Health Industry Co.,Ltd.,Jinan 250103,China;Neck-Shoulder and Lumbocrural Pain Hospital of Shandong First Medical University,Jinan 250062,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东省中医药研究院,山东济南250014 [3]山东宏济堂健康产业有限公司,山东济南250103 [4]山东第一医科大学附属颈肩腰腿痛医院,山东济南250062

出　　处：《药学研究》2024年第9期895-902,共8页Journal of Pharmaceutical Research

基　　金：山东省自然科学基金(No.ZR2020MH386);中央引导地方科技发展专项基金(No.YDZX2021117);山东省中央引导地方科技发展资金项目(No.YDZX20203700002055);山东省重点研发计划(科技示范工程)(No.2021SFGC1205)。

摘　　要：荧光标记技术是研究药物有效成分在体内分布、代谢路径、作用靶点等特征的有效手段,由于其操作简便、灵敏度高、特异性强、稳定性好、成本低等优势,被广泛应用于药物体内示踪研究中,对于阐明药物发挥作用的物质基础及其作用机制、改善药物的给药途径具有重要意义。本文综述了荧光示踪技术的原理及荧光染料与药物分子结合的方式,并讨论了该技术未来应用趋势,为药物成分体内示踪研究提供参考意义。Fluorescent labeling technology is an effective means to study the distribution,metabolic pathway,target and other characteristics of effective components of drugs in vivo.Because of its advantages of simple operation,high sensitivity,strong specificity,good stability and low cost,it is widely used in drug tracing research in vivo.It is of great significance to clarify the material basis and mechanism of drug action and improve the route of drug administration.In this paper,the principle of fluorescence tracing technology and the way of combining fluorescent dyes with drug molecules are reviewed,and the future application trend of this technology is discussed,which provides reference for the in vivo tracing research of drug components.

关 键 词：荧光标记 体内示踪 药物研究 荧光染料 活体成像 

分 类 号：R965.2[医药卫生—药理学]