羽毛针禾(Stipagrostis pennata)葡萄糖-6-磷酸-1-差向异构酶基因SpG6P1E的克隆与表达分析  

Cloning and expression analysis of Glucose-6-phospho 1 epimerase SpG6P1E in Stipagrostis pennata

作　　者：唐蕊 尹珊 陈凯露 李悦涛 程淋渊 王景儒 王斐[1,2,3] 李榕[1,2,3] 李鸿彬 Rui Tang;Shan Yin;Kailu Chen;Yuetao Li;Linyuan Cheng;Jingru Wang;Fei Wang;Rong Li;Hongbin Li(College of Life Science,Shihezi University,Shihezi 832003,Xinjiang,China;Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology,Shihezi University,Shihezi 832003,Xinjiang,China;Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education,Shihezi University,Shihezi 832003,Xinjiang,China)

机构地区：[1]石河子大学,生命科学学院,新疆石河子832003 [2]石河子大学,绿洲城镇与山盆系统生态兵团重点实验室,新疆石河子832003 [3]石河子大学,新疆植物药资源利用教育部重点实验室,新疆石河子832003

出　　处：《中国沙漠》2024年第4期126-136,共11页Journal of Desert Research

基　　金：国家自然科学基金项目(32060082);新疆生产建设兵团科技计划项目(2020BC002);石河子大学科技计划项目(KX008505);促进与加拿大、澳大利亚、新西兰及拉美地区科研合作与高层次人才培养项目。

摘　　要：葡萄糖-6-磷酸-1-差向异构酶(G6P1E)在植物生长发育和逆境胁迫响应中发挥着重要作用。本研究旨在通过克隆和分析沙漠植物羽毛针禾(Stipagrostis pennata)葡萄糖-6-磷酸-1-差向异构酶基因(SpG6P1E),为进一步探究其影响羽毛针禾根部沙套发育的机制及功能奠定基础。利用分子克隆技术从羽毛针禾中克隆获得一个SpG6P1E基因,该基因编码一个含有325个氨基酸的蛋白质,定位于细胞质,为亲水性稳定蛋白。序列及进化分析表明该基因含有一个保守性很高的醛糖异构酶(Aldose_epim)结构域,且与单子叶植物的直系同源基因亲缘性更高。亚细胞定位分析显示该基因定位于细胞质,参与胞质糖代谢过程。qRT-PCR结果显示SpG6P1E基因的表达与羽毛针禾沙套发育过程及可溶性糖含量具有较高的相关性,表明其对于沙套发育的重要作用;SpG6P1E基因的表达显著受到干旱、高温、盐等多种非生物胁迫的诱导,表明其对于胁迫响应的重要作用。蛋白互作网络及注释分析显示SpG6P1E可能通过参与糖合成和糖代谢等一系列过程进而影响羽毛针禾可溶性糖的含量。本研究结果为深入研究SpG6P1E基因的功能及其调控植物组织发育和适应逆境的机制奠定了基础。Glucose-6-phospho 1 epimerase(G6P1E)plays an important role in plant growth and development and stress response.The purpose of this study is to clone and analyze the expression characteristics of Stipagrosis penata SpG6P1E gene,and lay a foundation for further exploring the mechanism and function of its influence on the development of S.Pennata root sand trap.The full-length open reading frame sequence of S.pennata SpG6P1E gene was cloned by molecular cloning technology.SpG6P1E was cloned from the desert plant S.pennata,which includes a 325 amino acids.SpG6P1E was localized in the cytoplasm.Sequence and evolutionary analysis showed that this gene contains a aldose_epim domain,which is closely related to orthologous gene of monocotyledon plants.Subcellular localization analysis showed that the gene was located in cytoplasm and involved in cytoplasmic glucose metabolism.The results of qRT-PCR showed that SpG6P1E expression had a close link with rhizosheath development and soluble sugar content.The expression of SpG6P1E gene was significantly induced by abiotic stress.Protein interaction and annotation analysis indicated that SpG6P1E might affect the content of soluble sugar by participating in a series of processes such as sugar synthesis and sugar metabolism.The results of this study provide a foundation for in-depth investigation of the functions of G6P1E genes and the mechanisms by which they regulate plant tissue development and adaptation to stress.

关 键 词：羽毛针禾((Stipagrostis pennata)) 葡萄糖-6-磷酸-1-差向异构酶 SpG6P1E 生物信息学分析 亚细胞定位 沙套发育 非生物胁迫 

分 类 号：Q943[生物学—植物学]