交叉反应性糖类抗原决定簇抗体吸附剂在过敏原特异性IgE抗体检测中的应用分析  

Analysis of the effectiveness of cross-reactive carbohydrate antigen determinant antibody adsorbents in identifying allergen-specific IgE antibodies

作　　者：赵文玲 蔡蓓[1] 石楚琪 苏真珍[1] 冯伟华[1] Zhao Wenling;Cai Bei;Shi Chuqi;Su Zhenzhen;Feng Weihua(Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China;Allergy Center,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西医院实验医学科,成都610041 [2]四川大学华西医院过敏性疾病诊治中心,成都610041

出　　处：《中华预防医学杂志》2024年第9期1379-1383,共5页Chinese Journal of Preventive Medicine

摘　　要：本研究旨在探讨抗交叉反应性糖类抗原决定簇IgE抗体(anti-CCD IgE)对过敏原特异性IgE(sIgE)抗体检测的干扰,以及anti-CCD IgE吸附剂在过敏原sIgE检测中的应用价值。采用横断面研究,回顾性分析2020年10月至2021年5月就诊于四川大学华西医院并以免疫印迹法行过敏原sIgE检测样本2636例,其中过敏原sIgE阳性样本共计709例。随机收集过敏原sIgE阳性且交叉反应性糖类抗原决定簇(cross-reactive carbohydrate determinants,CCD)指示带为阳性的患者血清样本共46例,sIgE阳性但CCD指示带阴性血清样本1例,使用anti-CCD IgE吸附剂处理这些样本,再次进行过敏原sIgE检测,分析吸附前、后两次检测结果的差异。结果显示,本研究收集样本期间过敏原检测样本的CCD指示带阳性率为2.6%(69/2636)。47例样本经anti-CCD IgE吸附剂处理后,阳性率排名前三的过敏原sIgE从树组合2(柳树/杨树/榆树)、普通豚草、花生变更为尘螨组合、蟑螂和蟹。46例样本的anti-CCD IgE阳性结果全部转阴,sIgE抗体阳性总量下降了62.8%;阳性强度等级≥2的sIgE抗体经anti-CCD IgE吸附剂处理后阳性率显著降低,其中普通豚草阳性率降低达96.2%。阳性样本结果呈多sIgE抗体不同幅度下降,最多涉及11种抗体,最大降幅4级,强阳性(阳性强度等级≥4)sIgE抗体也有较高的转阴比例(25.0%~100%)。约60%样本的阳性sIgE抗体减少2种以上,17.4%样本的sIgE抗体转为全阴。1例anti-CCD IgE阴性样本处理后过敏原sIgE检测结果无变化。综上,普通豚草、葎草、树木组合2(柳树/杨树/榆树)等sIgE易受anti-CCD IgE影响,导致假阳性,经anti-CCD IgE吸附剂处理样本后可明显减少因anti-CCD IgE干扰引起的假阳性;anti-CCD IgE阳性样本使用anti-CCD IgE吸附剂进行预处理很有必要,可使实验室结果更准确,为过敏性疾病的诊断和预防提供参考依据。This study aimed to investigate the influence of anti-cross-reactive carbohydrate determinant IgE antibodies(anti-CCD IgE)on the detection of allergen-specific IgE(sIgE)antibodies,as well as the application value of anti-CCD IgE adsorbents in detecting allergen sIgE.In this cross-sectional study,a total of 2636 test samples from patients who received treatment in West China Hospital of Sichuan University and tested allergen sIgE using the western blot method from October 2020 to May 2021 were analyzed.In these samples,709 samples tested postive of allergen sIgE.46 stochastic venous serum samples that tested positive in both sIgE and anti-CCD IgE and 1 serum sample that tested positive in sIgE but negative in anti-CCD IgE were collected.These samples were processed by anti-CCD IgE adsorbents,followed by allergen sIgE detection.The difference between the two detection results before and after adsorption was analyzed.The allergen test results showed that the positive rate of anti-CCD IgE in samples was 2.6%(69/2636)during the period of sample collection.After treatment with anti-CCD IgE adsorbents,the top three allergen-sIgE of the positive rate changed from tree combination 2(willow/poplar/elm),common ragweed and peanut to dust mite combination,cockroach and crab.The positive anti-CCD IgE results of 46 samples all turned negative and the total positive sIgE antibody dropped by 62.8%;the positive rate of sIgE antibodies with the class result≥2 significantly decreased after treatment with anti-CCD IgE adsorbents,especially the positive rate of common ragweed dropped by 96.2%.The results of positive samples showed that multiple sIgE antibodies declined by different ranges,involving up to 11 antibodies with a maximum decline of 4 classes.Strongly positive sIgE antibodies(the class result≥4)also had a high conversion rate of negative(25.0%-100%).The positive sIgE antibodies in about 60% of the samples decreased by more than 2,and the sIgE antibodies in 17.4%of the samples turned completely negative.There was no chan

关 键 词：交叉反应性糖类抗原决定簇 抗交叉反应性糖类抗原决定簇IgE抗体 吸附剂 过敏原特异性IgE抗体 

分 类 号：R446.6[医药卫生—诊断学]