土茯苓中8个成分的含量测定及化学计量学分析  

Content determination and chemometric analysis of 8 components in Smilax glabra

作　　者：邹惠亮 王新财 陈柯 崔虹 孟令杰 刘百联[3] 陈敏 ZOU Huiliang;WANG Xincai;CHEN Ke;CUI Hong;MENG Lingjie;LIU Bailian;CHEN Min(Huzhou Institute for Food and Drug Control,Zhejiang Huzhou 313000,China;Institute of Life Sciences,Zunyi Medical University,Guizhou Zunyi 563000,China;Institute of Traditional Chinese Medicine and Natural Products,College of Pharmacy,Jinan University,Guangzhou 510632,China)

机构地区：[1]湖州市食品药品检验研究院,浙江湖州313000 [2]遵义医科大学生命科学研究院,贵州遵义563000 [3]暨南大学药学院中药及天然药物研究所,广州510632

出　　处：《中国药房》2024年第19期2378-2382,共5页China Pharmacy

基　　金：湖州市科技计划项目(No.2020GZ12)。

摘　　要：目的 为土茯苓药材的质量控制提供依据。方法 采用超高效液相色谱法同时测定不同产地20批(编号S1~S20)土茯苓中5-O-咖啡酰基莽草酸、新落新妇苷、落新妇苷、新异落新妇苷、异落新妇苷、黄杞苷、白藜芦醇、异黄杞苷的含量。采用化学计量学方法对20批土茯苓样品进行评价,并筛选影响土茯苓质量的差异标志物。结果 所测8个成分在各自检测质量浓度范围内线性关系良好(r≥0.999 6);精密度、重复性和稳定性(24 h)试验的RSD均小于2.00%(n=6);平均加样回收率为97.60%~106.40%,RSD均小于2.00%(n=6)。聚类分析结果显示,浙江产(S1~S5)和江西产(S6~S10)样品聚为一类,湖南产(S11~S15)样品聚为一类,云南产(S16~S20)样品聚为一类;主成分分析结果显示,前2个主成分可反映土茯苓中8个成分85.60%的信息量;偏最小二乘-判别分析结果显示,5-O-咖啡酰基莽草酸、落新妇苷、异落新妇苷和新落新妇苷的变量重要性投影值均大于1。结论 不同产地土茯苓中上述8个成分含量存在差异,5-O-咖啡酰基莽草酸、落新妇苷、异落新妇苷和新落新妇苷可能是影响土茯苓质量的差异标志物。OBJECTIVE To provide reference for Smilax glabra quality control.METHODS UPLC method was established to simultaneously determine the contents of 5-O-caffeoylshikimic acid,neoastilbin,astilbin,neoisoastilbin,isoastilbin,engelitin,resveratrol and isoengelitin in 20 batches of S.glabra from different areas(No.S1-S20).The quality evaluation of 20 batches of samples was performed by chemometrics;the differential biomarkers that affected the quality of S.glabra were screened.RESULTS The measured 8 components had good linear relationship within the range of measured concentration(r≥0.9996).RSDs of precision,repeatability and stability tests(24 h)were all lower than 2.00%(n=6).The average recoveries varied between 97.60%and 106.40%(RSDs were all lower than 2.00%,n=6).Cluster analysis showed that the samples produced in Zhejiang(S1-S5)and Jiangxi(S6-S10)were clustered into one category;the samples produced in Hunan(S11-S15)were clustered into one category;the samples produced in Yunnan(S16-S20)were clustered into one category.Principal component analysis showed that the first two principal components could represent 85.60%information of 8 components in S.glabra.Partial least squares-discriminant analysis showed that variable importance projection values of 5-O-caffeoylshikimic acid,astilbin,isoastilbin and neoastilbin were all greater than 1.CONCLUSIONS There are differences in the contents of the above 8 components in S.glabra from different origins;5-O-caffeoylshikimic acid,astilbin,isoastilbin and neoastilbin may be differential markers affecting the quality of S.glabra.

关 键 词：土茯苓 化学计量学 含量测定 超高效液相色谱法 5-O-咖啡酰基莽草酸 落新妇苷 异落新妇苷 新落新妇苷 

分 类 号：R917[医药卫生—药物分析学] R284.1[医药卫生—药学]