Molecular characterization of canine parvovirus type 2(CPV2)reveals a high prevalence of the CPV2c genotype among dogs sufering from diarrhea  

作　　者：Sajid Umar Di Gao Semin Kim Yixi Cheng Zhenkun Fang Qiu Zhongqi Weidong Yu Benjamin D.Anderson 

机构地区：[1]Global Health Research Center(GHRC),Duke Kunshan University,Kunshan,China [2]Division of Natural&Applied Sciences(DNAS),Duke Kunshan University,Kunshan,China [3]Swine Business Unit,MSD Animal Health,Shanghai,China [4]Simba Pet Hospital(Tinglin Park Branch),Maanshan Road,Kunshan,Suzhou 215335,Jiangsu Province,China [5]Play Pi Kangkang Pet Hospital,Kunshan City Development Zone,Kunshan,Suzhou 215300,Jiangsu Province,China [6]Department of Environmental and Global Health,College of Public Health and Health Professions,University of Florida,Florida,USA

出　　处：《Animal Diseases》2024年第3期198-206,共9页动物疾病（英文）

基　　金：Kunshan Municipal Government research funding(Grant Number:22KKSGR075).

摘　　要：Canine parvovirus 2(CPV-2)is a highly contagious virus in dogs that typically causes hemorrhagic enteritis and a high mortality rate in unvaccinated puppies.The genetic variability and antigenic diversity of CPV-2 hinder its efective prevention of infection by vaccination.To investigate the epidemiology and genetic characteristics of CPV-2 in China,rectal swabs from afected dogs were collected from diferent animal clinics in Kunshan from 2022 to 2023.Preliminary detection and capsid gene sequencing of CPV-2 were performed using previously described primers and protocols.The overall detection rate for CPV-2 was 16.5%(33/200).A signifcant association was found between the CPV-2-positivity and clinical signs,age,breed and vaccination status.Sequence analysis revealed the presence of CPV-2c genotypes in all positive samples,which were genetically similar to other Asian CPV-2c strains.Notably,four key mutations(A5G,F267Y,Y324I and Q370R)were detected in all isolates,and one novel mutation(I447M)was detected in three CPV-2 isolates.These mutations in the CPV-2 strains could impact vaccine efcacy and the efectiveness of the virus immune evasion.Surprisingly,no recombination events were observed between the identifed CPV-2c strains and reference strains from China.Our data revealed that amino acid residues 324,426 and 440 of VP2 may under strong selection pressure.This pattern of genetic variation in the CPV-2 lineage warrants continuous laboratory-based surveillance programs in other parts of China to better understand the pattern of seasonal distribution and association between emerging genotypes and the intensity of disease severity.

关 键 词：Canine parvovirus DOGS Epidemiology Genetic diversity Phylogenetic analysis 

分 类 号：S85[农业科学—兽医学]