骨质疏松症大鼠腰椎不同区域骨微结构变化的研究  

A study on the changes of bone microstructure in different regions of the lumbar spine in rats with osteoporosis

作　　者：佟金霖 刘昕 李圆圆 王玉瑶 李艳[1] 潘静华[1] 王少君[1] 赵宏艳[1] 刘红[1] TONG Jinlin;LIU Xin;LI Yuanyuan;WANG Yuyao;LI Yan;PAN Jinghua;WANG Shaojun;ZHAO Hongyan;LIU Hong(Bone and Joint Diseases Laboratory,Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院医学实验中心骨与关节疾病实验室,北京100700

出　　处：《中国骨质疏松杂志》2024年第9期1249-1254,1280,共7页Chinese Journal of Osteoporosis

基　　金：中国中医科学院科技创新工程项目(CI2021A00603);国家自然科学基金面上项目(82074299,81673841)。

摘　　要：目的基于Micro-CT方法探讨去卵巢手术(OVX)所致骨质疏松症大鼠腰椎不同区域骨量和骨微结构的差异。方法选用30只3月龄雌性SD大鼠,随机分为基础组、假手术组(Sham)和OVX组,分别在手术当天、手术后3月(3M)、手术后6月(6M)取第5腰椎,采用Micro-CT方法分析其远端、中部、近端、整体区域的骨量和骨微结构变化。结果(1)各组大鼠骨量和骨微结构的动态变化:Sham组大鼠各区域(除3M近端区域)BMD、BV/TV均随着时间明显增加(P<0.01,P<0.05),远端、近端、整体区域Tb.Th随着时间均明显增加(P<0.01),而中部区域Tb.N明显增多(P<0.05)。OVX组大鼠各区域(除3M近端区域)BMD、BV/TV均随时间显著降低(P<0.01,P<0.05)。OVX 3M,各区域Tb.N均较基础组明显减少(P<0.01,P<0.05),远端、中部、整体区域Tb.Sp均明显升高(P<0.01,P<0.05);OVX 6M,各个区域Tb.N明显减少(P<0.01),Tb.Sp明显升高(P<0.01,P<0.05),中部区域Tb.Th明显降低(P<0.05)。(2)OVX大鼠骨量和骨微结构变化的敏感性分析:OVX 3M,除近端外的3个区域BMD和BV/TV与Sham组相比均显著减少(P<0.01),远端、整体区域Tb.Th明显减少(P<0.05),远端、中部、整体区域Tb.N明显下降(P<0.01)、Tb.Sp明显升高(P<0.01,P<0.05);OVX 6M,各区域BMD和BV/TV与Sham组相比均显著减少(P<0.01),Tb.Th、Tb.N均明显下降(P<0.05,P<0.01),Tb.Sp明显升高(P<0.01)。结论在骨质疏松不同阶段,腰椎不同区域骨量和骨微结构变化趋势相同,但各指标变化的敏感性不同:骨质疏松后期各个区域均可以独立设为分析区域,但在早期选择远端区域或者整体区域更为合适。考虑分析操作方法的难易程度等因素,建议选择腰椎远端区域作为OVX所致骨质疏松症大鼠的分析区域。Objective To investigate whether there are differences in the bone mass and microstructure of the lumbar vertebrae in different regions of osteoporosis rats induced by ovariectomy(OVX)based on micro-CT.Methods Thirty female SD rats were randomly divided into basic group,Sham group,and OVX group.The 5th lumbar vertebrae were taken on the day of surgery,3 months(3M)after surgery,and 6 months(6M)after surgery.Micro-CT was used to analyze the bone mass and microstructure changes in the distal,middle,proximal,and whole regions.Results①Dynamic changes in bone microstructure of rats in each group:BMD and BV/TV in all regions(except for the 3M proximal region)of Sham group rats increased significantly over time(P<0.01,P<0.05),Tb.Th in the distal,proximal,and whole regions increased significantly over time(P<0.01),and Tb.N in the middle region increased significantly(P<0.05).In OVX rats at 3M and 6M,BMD and BV/TV significantly decreased over time in all regions(except for the 3M proximal region,P<0.01,P<0.05).Rats of OVX 3M showed a significant decrease in Tb.N in all regions compared to those in the basic group(P<0.01,P<0.05).Tb.SP in the distal,middle,and whole regions increased significantly(P<0.01,P<0.05).Rats of OVX 6M showed a significant decrease in Tb.Th in the middle region,compared to those in the basic group(P<0.05),a significant decrease in Tb.N in each region(P<0.01),and a significant increase in Tb.Sp(P<0.01,P<0.05).②Sensitivity analysis of bone microstructure changes in OVX rats:In rats of OVX 3M,except for the proximal region,BMD and BV/TV in the other three regions reduced significantly compared to those in the Sham group(P<0.01).Tb.Th of OVX rats in the distal and whole regions reduced significantly compared to those in the Sham group(P<0.05).Tb.N of OVX rats in the distal,middle,and whole regions reduced significantly(P<0.01)and Tb.Sp significantly increased(P<0.01,P<0.05).In rats of OVX 6M,BMD and BV/TV in all regions reduced significantly compared to those in the Sham group(P<0.01).Tb.Th and Tb.N

关 键 词：骨质疏松 去卵巢 MICRO-CT 腰椎 

分 类 号：R580[医药卫生—内分泌]