羊胎盘预防绝经后骨质疏松症的实验研究  

Prevention of postmenopausal osteoporosis induced by overiectomy in rats with sheep placebta

作　　者：宋慕格 杨世超 高玉海 魏振龙 王立强 安梓栋 陈克明 SONG Muge;YANG Shichao;GAO Yuhai;WEI Zhenlong;WANG Liqiang;AN Zidong;CHEN Keming(Clinical College of Traditional Chinese Medicine,GanSu University Traditional ChineseMedicine,Lanzhou 730000,China;Basic Medical Laboratory,No.940 Hospital of PLA Joint Logistic Support Force,Lanzhou 730050,China;Gansu Provincial Key Laboratory of Stem Cells and Gene Drug,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学中医临床学院,甘肃兰州730000 [2]联勤保障部队第九四〇医院基础医学实验室,甘肃兰州730050 [3]甘肃省干细胞与基因药物重点实验室,甘肃兰州730050

出　　处：《中国骨质疏松杂志》2024年第9期1292-1298,共7页Chinese Journal of Osteoporosis

基　　金：兰州市科技计划项目(2023-1-8);甘肃省自然科学基金(22JR11RA013);中国人民解放军联勤保障部队第九四〇医院实验室培育项目(2021yxky081)。

摘　　要：目的探讨口服羊胎盘粉能否预防绝经后骨质疏松症。方法建立骨质疏松大鼠模型,将大鼠随机分为假手术组(Sham)、模型组(OVX)、羊胎盘粉低(L)、中(M)、高剂量组(H)和炔雌醇组(EE)。每两周检测一次全身骨密度,1个半月后处死所有大鼠,称量并计算主要脏器系数,检测离体骨密度、血清骨代谢指标,进行Micro-CT扫描,观察骨组织形态变化。结果6周后OVX组大鼠的全身骨密度显著低于Sham组(P<0.01),但EE组未明显下降,羊胎盘三个组均显著高于OVX组(P<0.01)。离体股骨骨密度的变化呈相似趋势;Micro-CT扫描分析发现,羊胎盘三个组中只有M组的各项参数与Sham组和EE组接近,股骨脱钙后的组织病理学切片HE染色显示出与Micro-CT相似的结果;股骨三点弯曲试验发现,M组的最大载荷及强度均显著高于OVX组(P<0.01,P<0.05);血清骨代谢指标中,M组的骨形成指标BAP和PINP显著高于OVX组,骨吸收指标CTX-I和TRACP则显著低于OVX组。实验过程中,各组大鼠体重始终未出现显著性差异,心、肝、脾、肺、肾等主要脏器的外观无明显异常,器官系数除炔雌醇组的子宫外均无显著性差异。结论口服羊胎盘粉[300 mg/(kg?d)]可以通过促进骨形成、抑制骨吸双向作用,共同调节骨密度来预防切除卵巢大鼠发生骨质疏松,且羊胎盘粉没有产生使子宫内膜增厚的副作用。Objective To study whether oral sheep placenta powder canprevent postmenopausal osteoporosis.Methods:Established an osteoporosis model and randomly divide it into sham surgery group(Sham),model group(OVX),sheep fetal disc powder low(L),medium(M),high-dose group(H),and ethinylestradiol group(EE).The whole body bone density is measured every two weeks.After 1.5 months,weighed and calculated the main organ coefficients,detected the in vitro BMD and serum bone metabolism indexes,and performed Micro-CT scanning to observe the changes of bone tissue morphology.Results After 6 weeks,the overall bone density of the OVX group rats was significantly lower than that of the Sham group(P<0.01),but there was no significant decrease in the EE group.The three groups of sheep fetal discs were significantly higher than those of the OVX group(P<0.01).The changes in bone mineral density of the isolated femur showed a similar trend,but there was no significant difference between the Low group and OVX(P>0.05).Micro-CT scan analysis showed that among the three groups of sheep placenta,only the parameters of group M weresimilar to those of Sham group and EE group,and HE staining of histomatological sections of femur after decalcification showed similar results to those of Micro-CT.The three-point bending test of the femur found that the maximum load and strength of the M group were significantly higher than those of the OVX group(P<0.01,P<0.05).In serum bone metabolism indicators,the bone formation indicators BAP and PINP in the M group were significantly higher than those in the OVX group,while the bone resorption indicators CTX-I and TRACP were significantly lower than those in the OVX group.During the experiment,there were no significant differences in body weight among all groups,no obvious abnormalities in the appearance of the heart,liver,spleen,lung,kidney and other major organs,no significant differences in organ coefficients except the uterus of the ethoestradiol group,and no significant abnormalities were found in pathological

关 键 词：羊胎盘粉 切卵巢大鼠 骨密度 骨质疏松症 骨形成 

分 类 号：R681[医药卫生—骨科学]