一个新的水稻黄绿叶突变体ygl-9108的鉴定与基因定位  

Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice

作　　者：刘晓敏 孙志广 迟铭[1] 邢运高 徐波[1] 李景芳 刘艳[1] 卢百关 王宝祥[1] 徐大勇[1] LIU Xiaomin;SUN Zhiguang;CHI Ming;XING Yungao;XU Bo;LI Jingfang;LIU Yan;LU Baiguan;WANG Baoxiang;XU Dayong(Lianyungang Academy of Agricultural Sciences/Jiangsu Collaborative Innovation Center for Modern Corp Production,Lianyungang,Jiangsu 222000)

机构地区：[1]连云港市农业科学院/江苏省现代作物生产协同创新中心,江苏连云港222000

出　　处：《中国农学通报》2024年第26期22-29,共8页Chinese Agricultural Science Bulletin

基　　金：国家现代农业产业技术体系建设专项“适合轻简化生产的水稻品种培育”(CARS-01-01A);江苏省种业振兴揭榜挂帅项目“抗稻瘟病外观透明优质食味早熟晚粳稻新品种培育”(JBGS[2021]039);连云港市财政专项“水稻粉质突变体m149表型分析及基因定位”(QNJJ2311),“连云港穞稻优异基因的挖掘”(QNJJ2203)。

摘　　要：本研究旨在对水稻黄绿叶突变体ygl-9108进行表型分析和基因定位,为后续图位克隆该叶色相关基因奠定基础。以黄绿叶突变体ygl-9108为试材,利用突变体ygl-9108和‘五山丝苗’杂交的F2群体进行基因定位。结果表明,与野生型相比,突变体的株高、有效穗数、穗长、每穗粒数、结实率、粒宽、千粒重和单株谷重均显著下降,下降比例分别为21.5%、21.2%、14.6%、19.2%、11.0%、10.2%、12.9%和52.2%。透射电镜观察显示,突变体叶肉细胞的叶绿体数量大量减少,类囊体结构异常。遗传分析表明,突变体的表型由一个隐性核基因控制。利用图位克隆方法,ygl-9108被定位于水稻第11号染色体两InDel标记11Y39和11Y45之间,物理距离约为147 kb,该区间内未见有叶色相关基因的报道,表明ygl-9108是一个新的黄绿叶调控基因。本研究结果为ygl-9108的克隆和功能分析奠定了坚实的基础。The research aims to lay the foundation for map-based cloning of the leaf color related gene by analyzing the phenotypic characteristics of a rice yellow-green leaf mutant ygl-9108 and mapping the mutant locus.A yellow-green leaf mutant,named as ygl-9108,was selected as experimental material,and the gene was mapped by using the F2 population derived from the cross between the ygl-9108 mutant and‘Wushansimiao’.The results showed that the plant height,effective panicle number,panicle length,grain number per panicle,seed setting rate,grain width,1000-grain weight,and grain weight per plant of the ygl-9108 mutant were all significantly reduced by 21.5%,21.2%,14.6%,19.2%,11.0%,10.2%,12.9%,and 52.2%,respectively,compared with those of the wild type.The observation by transmission electronic microscope(TEM)demonstrated that the number of chloroplasts in mesophyll cells of ygl-9108 plants was greatly reduced,and the lamellar structure of chloroplasts was abnormal.Genetic analysis revealed that the phenotype of mutant was controlled by a single recessive nuclear gene.Using a map-based cloning strategy,ygl-9108 was mapped to a 147 kb region on chromosome 11 between two InDel markers,11Y39 and 11Y45.No gene related to leaf color has been reported previously in this interval,indicating that ygl-9108 is a new leaf color-related gene.This study lays a solid foundation for further research on gene cloning and function analysis of ygl-9108.

关 键 词：水稻 黄绿叶突变体 ygl-9108 表型鉴定 农艺性状 基因定位 透射电镜 叶绿体 隐性核基因 图位克隆 

分 类 号：S511[农业科学—作物学]