党参资源再生体系种子灭菌方法研究  

Regeneration System of Codonopsis pilosula Resources:Seed Sterilization Methods

作　　者：王秋宝 马宏斌 王耀琴 田洪岭 刘月贤 裴帅帅[1] 左宪强 WANG Qiubao;MA Hongbin;WANG Yaoqin;TIAN Hongling;LIU Yuexian;PEI Shuaishuai;ZUO Xianqiang(Institute of Economic Crops,Shanxi Agricultural University,Fenyang,Shanxi 032200;Shanxi Agricultural University,Taiyuan 030000;Wenxi Agricultural and Rural Bureau,Wenxi,Shanxi 043800)

机构地区：[1]山西农业大学经济作物研究所,山西汾阳032200 [2]山西农业大学,太原030000 [3]闻喜县农业农村局,山西闻喜043800

出　　处：《中国农学通报》2024年第27期40-45,共6页Chinese Agricultural Science Bulletin

基　　金：山西农业大学学术恢复项目“远志、黄芪、党参种质资源征集及鉴定评价”(2020xshf53);山西省农业种质资源保护利用项目“山西中药材种质资源收集、利用与保护”(sxzyk202306)。

摘　　要：党参作为山西省道地药材,其品质及疗效享誉全国,本研究旨在建立一套高效的党参种子再生体系,以保护和利用山西省道地药材党参的优质种质资源。试验以党参种子为试材,采用4种不同处理方法,对党参种子进行隔离和包裹后,分别使用升汞和次氯酸钠两种不同的灭菌剂处理。通过观察不同处理对种子的损伤情况,评估各种方法的效果,以确定最佳的灭菌方法。结果表明,在不同灭菌处理下,种子污染率在0%~92.7%之间,成活率在0.8%~91.3%之间。结合污染率与成活率,筛选出了处理29为结果最佳,处理30次之。采用方法4包裹种子后,经60~120 s的升汞灭菌处理,可以获得最佳的灭菌效果,此时种子的成活率为82.00%~91.3%,污染率为2.17%~3.83%。该试验成功建立了适合党参再生种子体系的灭菌方法,为党参生物技术育种工作奠定了基础。As an authentic medicinal material in Shanxi Province,Codonopsis pilosula(Franch.)Nannf.is wellknown throughout the country for its quality and curative effect.In order to effectively protect the high-quality germplasm resources of Codonopsis pilosula,an efficient regeneration system of Codonopsis pilosula was constructed.In this experiment,Codonopsis pilosula seeds were used as test materials,using 4 different treatment methods,after isolation and wrapping,two different sterilizers were used to treat mercury and sodium hypochlorite,respectively,and the damage to the seeds by different treatments was observed to determine the best sterilization method.The results showed that under different sterilization treatments,the seed contamination rate was 0-92.7%,and the survival rate was 0.8%-91.3%.Based on the combination of pollution rate and survival rate,treatment 29 was selected as the best result and followed by treatments 30.After wrapping the seeds in method 4,the best effect was achieved after 60-120 s of mercury sterilization,and the survival rate of the seeds was 82.00%-91.3%,while the contamination rate was 2.17%-3.83%.This experiment establishes a seed sterilization method suitable for the regeneration system of Codonopsis pilosula and lays the foundation for biotechnological breeding of Codonopsis pilosula.

关 键 词：党参 种子 污染率 成活率 愈伤组织 再生体系 灭菌方法 

分 类 号：S317[农业科学—作物栽培与耕作技术]