龙血树组织诱导快速繁殖技术研究  

Tissue Culture for Rapid Propagation of Dracaena cambodiana

作　　者：郑焙华 Zheng Beihua(Quanzhou Forestry Bureau,Quanzhou,Fujian 362000,China)

机构地区：[1]泉州市林业局,福建泉州362000

出　　处：《东南园艺》2024年第3期177-182,共6页Southeast Horticulture

基　　金：福建省林业科技项目(2024FKJ09);泉州市科技计划项目(2020N026)。

摘　　要：【目的】探究不同消毒方法和激素配比对龙血树组培快繁技术的影响,为建立龙血树组培快繁体系提供参考。【方法】以‘太空’和‘68#’2个龙血树品种的外植体为试材,探讨不同的灭菌时间、激素配比及浓度对龙血树不定芽诱导、增殖和生根的影响。【结果】研究表明龙血树无菌材料的获得,适宜采用带顶芽茎段,先用75%酒精消毒20 s,再用0.1%升汞溶液消毒8 min,消毒效果最佳。MS+5.0 mg/L 6-BA+3.0 g/L花宝为最佳不定芽诱导培养基;MS+2.0 mg/L 6-BA+0.1 mg/L NAA为最佳增殖培养基,太空和68#增殖系数分别达5.35和4.87;MS+1.0 mg/L NAA为最佳生根培养基,太空和68#的生根率均高于99%,生根数分别达到9.2和11.2条。【结论】建立以带顶芽茎段为外植体的龙血树组织培养快繁体系,为龙血树工厂化生产和遗传转化等研究奠定良好基础。【Objective】 The objective of this study is to explore the effects of different disinfection methods and hormone ratios on rapid propagation technology of Dracaena cambodiana tissue culture,and to provide references for establishing a rapid propagation system of D. cambodiana tissue culture. 【Method】 The effects of different sterilization times,plant hormone ratios and concentrations on the induction,proliferation,and rooting of adventitious buds were explored using‘Space'and‘68 #'as materials. 【Result】 The results showed that the aseptic materials could be obtained using the stem with apical bud when it was disinfected by 75% alcohol for 20seconds and 0.1% mercuric chloride for 8 minutes,achieving the best disinfection effect. The optimal medium for inducing adventitious buds was MS + 5.0 mg/L 6-BA + 3.0 g/L Huabao;the optimal proliferation medium was MS +2.0 mg/L 6-BA + 0.1 mg/L NAA,and the proliferation coefficients of‘Space'and‘68 #'reached 5.35 and 4.87,respectively;the optimal rooting medium was MS + 1.0 mg/L NAA and the rooting rates of‘Space'and‘68 #'were both above 99%,with rooting numbers of 9.2 and 11.2,respectively. 【Conclusion】 The establishment of tissue culture and rapid propagation system of D. cambodiana with apical bud stem as explants will lay a good foundation for the industrial production and genetic transformation of D. cambodiana.

关 键 词：龙血树 植物生长调节剂 组织培养 快速繁殖 

分 类 号：S432[农业科学—植物病理学]