基于PPARγ/NF-κB信号通路探讨四妙勇安汤对脂肪细胞炎症的作用机制研究  

A study on the mechanism of action of Simiaoyong′an Decoction on adipocyte inflammation based on PPARγ/NF⁃κB signaling pathway

作　　者：张晴玥 许心蕊 冯露 邵瑞洁 王添钰 王霄 娄利霞[1] 吴爱明[1] 聂波[1,2] ZHANG Qingyue;XU Xinrui;FENG Lu;SHAO Ruijie;WANG Tianyu;WANG Xiao;LOU Lixia;WU Aiming;NIE Bo(Dongzhimen Hospital of Beijing University of Chinese Medicine,Key Laboratory of Chinese Internal Medicine of Ministry of Education/Beijing,Beijing 100700,China;School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学东直门医院,中医内科学教育部和北京市重点实验室,北京100700 [2]北京中医药大学中药学院,北京100029

出　　处：《海南医学院学报》2024年第18期1361-1369,共9页Journal of Hainan Medical University

基　　金：国家自然科学基金面上项目(81874446、82274488)。

摘　　要：目的:从PPARγ/NF-κB信号通路探讨四妙勇安汤对脂多糖(lipopolysaccharide,LPS)诱导的脂肪细胞炎症的影响及作用机制。方法:采用LPS诱导成熟脂肪细胞制备脂肪细胞炎症模型。给予四妙勇安汤冻干粉干预24 h后观察脂肪细胞炎症信号通路蛋白的表达,给予PPARγ抑制剂GW9662干预后明确四妙勇安汤是否是通过关键因子PPARγ发挥抗炎作用。CCK-8法检测四妙勇安汤对细胞活力的影响;蛋白质免疫印迹法(Western blot,WB)检测细胞内炎性因子IL-6、NLRP3及相关调控因子PPARγ、NF-κB p65、LXRα和GLUT4的蛋白表达。结果:CCK-8检测结果显示四妙勇安汤冻干粉在浓度为0~500μg/mL范围内对细胞活力无显著影响。WB结果显示:经1μg/mL LPS刺激脂肪细胞造模,与对照组相比,模型组细胞内炎症因子IL-6、NLRP3蛋白表达增加,PPARγ蛋白表达降低、NF-κB p65蛋白表达增加,LXRα、GLUT4蛋白表达均降低;与模型组相比,四妙勇安汤组细胞内炎症因子IL-6、NLRP3蛋白表达降低,且呈剂量依赖性变化趋势,并明确四妙勇安汤干预最佳药物浓度为250μg/mL;给予中剂量四妙安勇汤干预后,脂肪细胞内PPARγ、LXRα、GLUT4蛋白表达增加,NF-κB蛋白表达减少,给予PPARγ抑制剂GW9662后四妙勇安汤调控关键因子和抗炎作用相应减弱。结论:四妙勇安汤通过PPARγ/NF-κB信号通路发挥抑制脂肪细胞炎症的作用。Objective:To investigate the effect of Simiaoyong'an Decoction on lipopolysaccharide(LPS)-induced inflammation in adipocytes and its underlying mechanism through the PPARγ/NF-κB signaling pathway.Methods:An adipocyte inflammation model was established by inducing mature adipocytes with LPS.The expression of proteins involved in the adipocyte inflammatory signaling pathway was evaluated after 24 h of intervention by administration of freeze-dried powder of Simiaoyong’an Decoction.The effect of Simiaoyong'an Decoction on adipocytes activity was detected by CCK-8 assay.The expression of IL-6,NLRP3,PPARγ,NF-κB p65,LXRαand GLUT4 by Western blot(WB).Results:CCK-8 results showed that freeze-dried powder of Simiaoyong’an Decoction had no significant effect on the viability of adipocytes in the range of 0~500μg/mL.The WB results re-vealed that following LPS intervention,the model group exhibited increased intracellular inflammatory factors IL-6 and NLRP3 expression,decreased PPARγexpression,increased NF-κB p65 expression,and decreased LXRαand GLUT4 expression com-pared to the control group.Compared with the model group,Simiaoyong’an Decoction dose-dependently reduced IL-6 and NL-RP3 expression.Additionally,the expression of PPARγ,LXRαand GLUT4 increased,while the expression of NF-κB de-creased.The anti-inflammatory effect of Simiaoyong'an Decoction was correspondingly weakened after administration of the PPARγinhibitor GW9662.Conclusion:Simiaoyong'an Decoction exerts an inhibitory effect on adipocyte inflammation via the PPARγ/NF-κB signaling pathway.

关 键 词：四妙勇安汤 PPARΓ NF-ΚB 脂肪细胞 炎症 

分 类 号：R285[医药卫生—中药学]