益生乳酸菌促进亮斑扁角水虻幼虫生长及蛋白积累研究  

Probiotic lactic acid bacteria promote the growth and protein accumulation of Hermetia illucens L.larvae

作　　者：禹铭洋 邵明英[2] 余永强 张珈 蔡珉敏[1] 郑龙玉[1] 张吉斌[1] YU Ming-Yang;SHAO Ming-Ying;YU Yong-Qiang;ZHANG Jia;CAI Min-Min;ZHENG Long-Yu;ZHANG Ji-Bin(National Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,College of Life Science and Technology,National Engineering Research Center for Microbial Pesticides,Huazhong Agricultural University,Wuhan 430070,China;Hainan Vocational University,Haikou 570216,China)

机构地区：[1]农业微生物资源发掘与利用全国重点实验室,湖北洪山实验室,华中农业大学生命科学技术学院,微生物农药国家工程研究中心,武汉430070 [2]海南职业技术学院,海口570216

出　　处：《环境昆虫学报》2024年第5期1094-1103,共10页Journal of Environmental Entomology

基　　金：海南省重点研发项目(ZDYF2023XDNY033);湖北省重点研发计划(2021BBA258)。

摘　　要：亮斑扁角水虻Hermetia illucens L.幼虫能够转化有机废弃物如餐厨垃圾等,获得虫体蛋白可以生产畜禽和水产饲料,虫粪可作为优质有机肥。益生微生物在亮斑扁角水虻幼虫处理有机废弃物过程中扮演着至关重要的角色,然而乳酸菌对亮斑扁角水虻高效处理有机废弃物的促进作用却鲜有研究。本研究以亮斑扁角水虻幼虫为研究对象,通过添加不同的乳酸菌到亮斑扁角水虻幼虫处理人工饲料的体系中,评估不同乳酸菌菌株对亮斑扁角水虻幼虫转化人工饲料的影响,以筛选出能够提高亮斑扁角水虻幼虫存活率、转化率以及促进幼虫蛋白积累效果显著的乳酸菌并定义其为益生乳酸菌。通过将乳酸菌添加到亮斑扁角水虻幼虫转化人工饲料体系,初步筛选出3株亮斑扁角水虻益生乳酸菌L4,L7,L8。经过形态学观察、生理生化鉴定及16S rDNA序列分析后,确定L4为短促生乳杆菌,L7为啤酒促生乳杆菌,L8为植物乳植物杆菌。与空白对照组相比,益生乳杆菌L4,L7,L8的添加可以显著提高亮斑扁角水虻幼虫鲜重5.93%,6.41%,9.43%;亮斑扁角水虻转化率也分别提高了8.01%,7.18%,13.60%;物料减少率则分别提高了3.47%,4.75%,6.28%。更重要的是,L4,L7,L8对亮斑扁角水虻幼虫蛋白积累具有显著促进作用。与空白对照组相比,亮斑扁角水虻幼虫粗蛋白含量分别显著提高了5.14%,3.13%,4.70%;幼虫蛋白总产量分别显著提高了14.40%,14.84%,17.63%。综上,本研究筛选、鉴定并评估了对亮斑扁角水虻幼虫具有益生作用的乳杆菌,不仅能提高亮斑扁角水虻幼虫对人工饲料的转化率,获得更多的虫体生物质,而且能够促进亮斑扁角水虻幼虫的蛋白积累提高虫体蛋白质含量,获得高品质的昆虫蛋白。研究结果对揭示亮斑扁角水虻与肠道微生物协同代谢营养物质的机制具有重要意义,并且在亮斑扁角水虻大规模转化有机废弃物中应用潜力巨大Hermetia illucens L.larvae can convert organic waste such as food waste.They produce insect protein,which can be used for livestock and aquaculture feed.Meanwhile,their frass serves as high-quality organic fertilizer.Probiotic microbes play a vital role in the processing of organic waste by the black soldier fly larvae(BSFL).However,the role of lactic acid bacteria in promoting the efficient processing of organic waste by black soldier fly had rarely been studied.This study focused on the black soldier fly larvae and evaluated the impact of different lactic acid bacteria strains added to the artificial feed system for these larvae.The goal was to identify lactic acid bacteria that enhance larval survival rate,conversion efficiency,and protein accumulation,designating them as probiotic lactic acid bacteria.By adding lactic acid bacteria to the black soldier fly larvae’s artificial feed system,three candidate probiotic lactic acid bacteria strains(L4,L7,and L8)were preliminarily screened.After morphological observation,physiological and biochemical identification,and 16S rDNA sequence analysis,L4 was identified as Levilactobacillus brevis,L7 as Levilactobacillus cerevisiae,and L8 as Lactiplantibacillus plantarum.Compared to the control group,the addition of probiotic lactobacilli L4,L7,and L8 significantly increased the fresh weight of black soldier fly larvae by 5.93%,6.41%,and 9.43%,respectively.Additionally,the conversion rate of the larvae improved by 8.01%,7.18%,and 13.60%,while the reduction in feed material was enhanced by 3.47%,4.75%,and 6.28%.Importantly,L4,L7,and L8 demonstrated a significant promotion effect on protein accumulation in black soldier fly larvae.Compared to the control group,the crude protein content of the larvae increased significantly by 5.14%,3.13%,and 4.70%,respectively.Additionally,the total protein yield of the larvae increased significantly by 14.40%,14.84%,and 17.63%.In summary,this study screened,identified,and evaluated lactic acid bacteria with probiotic effects on black soldi

关 键 词：亮斑扁角水虻 益生乳杆菌 协同转化 生长 蛋白积累 

分 类 号：Q968.1[生物学—昆虫学] S89[农业科学—特种经济动物饲养]