基于转录组测序技术研究黑水虻幼虫对锌离子胁迫的响应  

Response of Hermetia illucens L.to zinc ion stress based on transcriptome sequencing technology

作　　者：徐晨露 李笑驹 孙虹霞 夏嫱 XU Chen-Lu;LI Xiao-Ju;SUN Hong-Xia;XIA Qiang(Zhuhai Campus of Zunyi Medical University,Zhuhai 519041,Guangdong Province,China)

机构地区：[1]遵义医科大学珠海校区,广东珠海519041

出　　处：《环境昆虫学报》2024年第5期1113-1126,共14页Journal of Environmental Entomology

基　　金：国家自然科学基金(31260528,32060127)。

摘　　要：黑水虻Hermetia illucens L.是世界范围内重要资源昆虫,过量锌离子(Zn^(2+))胁迫会对其幼虫的生长发育产生影响,而转录组分析有助于揭示Zn^(2+)胁迫影响黑水虻幼虫发育的分子机制。本试验以不同浓度Zn^(2+)(75、150、300、1200 mg/kg)对黑水虻幼虫进行连续5代胁迫,利用Illumina HiSeq 4000高通量测序平台对第5代6龄幼虫转录组进行测序及分析。结果显示,Zn^(2+)胁迫影响了第5代黑水虻幼虫的基因表达水平,75、150、300和1200 mg/kg Zn^(2+)处理幼虫中上调/下调的差异表达基因分别是1293/1839、1277/2306、1080/1966和1444/2170个;GO富集分析结果表明,差异表达基因在细胞过程中所占比例最大,细胞组分中主要是细胞和细胞成分,分子功能中主要为催化活性和细胞结合;KEGG富集差异分析显示,75 mg/kg Zn^(2+)胁迫下差异表达基因主要富集在免疫相关反应、免疫相关信号传导途径及脂类物质代谢,300 mg/kg Zn^(2+)处理组差异表达基因主要富集在氨基酸代谢和碳水化合物代谢;1200 mg/kg Zn^(2+)胁迫下氨基酸和脂类代谢、免疫相关反应和生长均受到严重影响,这些富集的与上述有关的差异基因大多表达下调。本研究表明,Zn^(2+)胁迫影响了黑水虻幼虫基因表达,高浓度Zn^(2+)胁迫基因表达影响尤为显著,主要表现在细胞过程、催化活性和结合的基因表达下调;高浓度Zn^(2+)胁迫还导致了黑水虻幼虫免疫系统、环境相关信号传导途径和脂质代谢等的紊乱。Hermetia illucens is a globally significant resource insect.Excessive zinc ion(Zn^(2+))stress can adversely affect the growth and development of its larvae.Transcriptome analysis provides insights into the molecular mechanisms underlying the impact of Zn^(2+)stress on black hydra larvae.In this study,successive generations of Black Hydra larvae were subjected to different concentrations of Zn^(2+)(75,150,300,1200 mg/kg)stress,and the transcriptome of 6th instar larvae from the 5th generation was sequenced and analyzed by Illumina HiSeq 4000 high-throughput sequencing platform.The results showed that Zn^(2+)stress affected the gene expression in the fifth generation H.illucens larvae.Differentially expressed genes were observed in larvae treated with 75,150,300 and 1200 mg/kg Zn^(2+),with counts ranging from 1293/1839 to 1444/2170 respectively.GO enrichment analysis indicated that differentially expressed genes primarily affected cellular processes and components such as cells and cell components;while molecular functions mainly involved catalytic activity and cell binding.KEGG enrichment differential analysis revealed that under 75 mg/kg Zn^(2+)stress,the differentially expressed genes were predominantly enriched in immune-related reactions,immune-related signal transduction pathways,and lipid metabolism.Conversely,under 300 mg/kg Zn^(2+)treatment,the differentially expressed genes were primarily enriched in amino acid metabolism and carbohydrate metabolism.Under 1200 mg/kg Zn^(2+)stress,there was a significant impact on amino acid and lipid metabolism,immune-related reactions,and growth in H.illucens larvae.Most of these enriched differential genes associated with the aforementioned processes exhibited down-regulation.Therefore,Zn^(2+)stress exerted an influence on the expression of differential genes in H.illucens larvae;particularly at high concentrations of Zn^(2+),which mainly affected cell processes,catalytic activity,combined gene expression resulting mostly in down-regulation.Additionally,high concentrati

关 键 词：黑水虻幼虫 锌胁迫 转录组 差异基因 信号通路 

分 类 号：Q963[生物学—昆虫学] S89[农业科学—特种经济动物饲养]