二化螟盘绒茧蜂茧丝纤维的形态结构与理化性能  

Morphological structure and physicochemical properties of silk fibers from the cocoons of the parasitoid wasp,Cotesia chilonis(Matsumura)(Hymenoptera:Braconidae)

作　　者：李小雨 胡蝶 黄廷莉 姚洪渭[2] 蒋彩英[1] LI Xiao-Yu;HU Die;HUANG Tin-Li;YAO Hong-Wei;JIANG Cai-Ying(Zhejiang Sci-Tech University,College of Life Sciences and Medicine,Hangzhou 310018,China;Zhejiang University,Institute of Insect Sciences,Zhejiang Provincial Key Lab of Biology of Crop Pathogens and Insects,State Key Laboratory of Rice Biology,Hangzhou 310058,China)

机构地区：[1]浙江理工大学生命科学与医药学院,杭州310018 [2]浙江大学昆虫科学研究所,浙江省作物病虫生物学重点实验室,水稻生物学国家重点为实验室,杭州310058

出　　处：《环境昆虫学报》2024年第5期1255-1260,共6页Journal of Environmental Entomology

摘　　要：本文通过扫描电镜(SEM)、氨基酸分析、傅立叶红外光谱(FTIR)、X射线晶体衍射(XRD)和热重分析(TG)等技术对二化螟盘绒茧蜂Cotesia chilonis茧丝纤维的微观形态、氨基酸类别与组成、二级结构和热稳定性等作了分析。结果表明,二化螟盘绒茧蜂的茧丝纤维表面较为粗糙,直径为1.89±0.04μm。茧丝纤维主要由碳(60.67%)、氮(21.17%)和氧(17.22%)等元素组成,其丝蛋白主要成分包括天冬氨酸/天冬酰胺、丝氨酸和丙氨酸等;蛋白二级结构主要为β-折叠;其峰值降解温度为332.27℃±2.58℃。本文研究揭示了二化螟盘绒茧蜂茧丝纤维的结构特征与理化性能,为拓展该蜂的应用领域、开发性能优越的非蚕丝蛋白以及设计改造新型丝蛋白纤维提供基础。In this paper,the morphological structure,amino acid composition,secondary structure and thermal stability of silk fibers from the cocoons of the parasitoid wasp,Cotesia chilonis(Matsumura)(Hymenoptera:Braconidae)were analyzed by the scanning electron microscopy(SEM),amino acid analysis,Fourier transform infrared spectroscopy(FTIR),X-ray crystallography(XRD)and thermogravimetric analysis(TG).The results showed that the surface of the silk fiber from C.chilonis cocoons was relatively rough,and its diameter was 1.89±0.04μm.It was mainly composed of elements such as carbon(60.67%),nitrogen(21.17%),and oxygen(17.22%).The protein components of C.chilonis cocoons mainly included aspartic acid/asparagine,serine and alanine.The secondary structure of silk proteins from C.chilonis cocoons was mainlyβ-sheet,and its peak degradation temperature was 332.27±2.58℃.This study reveals the ultrastructural characteristics and physicochemical properties of silk fibers from C.chilonis cocoons,which provides a basis for expanding the application field of C.chilonis,developing non-worm silk proteins with superior performance,and designing and transforming new silk protein fibers.

关 键 词：二化螟盘绒茧蜂 茧 丝纤维 形态结构 理化性能 

分 类 号：Q964[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]