臭椿canthin-6-one对禾谷镰刀菌的抑菌作用  

Antifungal effect of canthin-6-one from Ailanthus altissima on Fusarium graminearum

作　　者：赵美荣 李永春 李春颖 ZHAO Mei-rong;LI Yong-chun;LI Chun-ying(College of Chemistry and Life Science,Chifeng University,Chifeng 024000,Inner Mongolia,China)

机构地区：[1]赤峰学院化学与生命科学学院,内蒙古赤峰024000

出　　处：《湖北农业科学》2024年第9期84-88,共5页Hubei Agricultural Sciences

基　　金：内蒙古自治区高等学校科学研究项目(NJZY21137,NJZZ22150);赤峰学院一流学科建设项目(CFXYYLXKB202102);蒙东地区生物资源开发利用创新团队项目(FXYKYCXTD202008)。

摘　　要：为进一步研究臭椿(Ailanthus altissima)canthin-6-one生物碱对禾谷镰刀菌(Fusarium graminearum)的抑菌效果及其作用机制,采用微量稀释法、菌丝生长抑制法测定其抑菌活性,并通过测定胞外相对电导率、内容物释放量、丙二醛含量、过氧化氢含量以及保护酶活性,从细胞膜角度分析其抑菌作用机制。结果显示,canthin-6-one对禾谷镰刀菌具有显著的抑菌作用,其最小抑菌浓度(MIC)为8.0μg/mL,半数有效浓度(EC_(50))为1.85μg/mL。处理后的菌体胞外相对电导率和内容物释放量显著增加,同时菌体丙二醛和过氧化氢含量亦有显著升高。这表明canthin-6-one引起了禾谷镰刀菌细胞膜氧化损伤,导致膜完整性丧失及透性增强,从而抑制菌丝生长。To further study the fungicidal effect and mechanism of canthin-6-one alkaloid from Ailanthus altissima on Fusarium gra-minearum,the inhibitory activity was determined by microdilution and mycelial growth inhibition methods.The antifungal mechanism of canthin-6-one was analyzed from the point of view of cell membrane by detecting the extracellular relative conductivity,content re-lease,malondialdehyde content,hydrogen peroxide content and protective enzyme activity.The results showed that canthin-6-one had a significant inhibitory effect on Fusarium graminearum,with a minimum inhibitory concentration of 8.0μg/mL and a half-effec-tive concentration of 1.85μg/mL.The extracellular relative conductivity and the content release amount of the treated mycelia were sig-nificantly increased,while malondialdehyde and hydrogen peroxide contents were also significantly elevated.It was indicated that can-thin-6-one induced oxidative damage to the cell membrane of Fusarium graminearum,resulting in the loss of membrane integrity and increased permeability,thus inhibiting mycelial growth.

关 键 词：臭椿(Ailanthus altissima) 禾谷镰刀菌(Fusarium graminearum) 生物碱 抑菌作用 细胞膜 

分 类 号：S482.2[农业科学—农药学]