多倍体水稻DHF-CBS基因的抗旱性鉴定  

Identification of drought resistance of polyploid Oryza sativa L.DHF-CBS gene

作　　者：葛杰[1] 何玉池[2] 展帆 李之龙 GE Jie;HE Yu-chi;ZHAN Fan;LI Zhi-long(College of Life Sciences/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University,Urumqi 830052,China;School of Life Sciences,Hubei University,Wuhan 430062,China)

机构地区：[1]新疆农业大学生命科学学院/农业生物技术重点实验室,乌鲁木齐830052 [2]湖北大学生命科学学院,武汉430062

出　　处：《湖北农业科学》2024年第9期216-220,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(32160494);新疆农业大学大学生创新创业训练计划项目(dxscx2022068)。

摘　　要：为探究干旱胁迫下DHF-CBS基因调控多倍体水稻(Oryza sativa L.)的抗旱机制,选取2种水稻品种,分别为多倍体植株中花-11(DHF-CBS基因)、突变体植株UC-32(不表达DHF-CBS基因),对2种水稻的GUS化学染色结果、组织含水量、脱落酸(ABA)含量、硫代巴比妥酸活性物质(TBARS)含量、谷胱甘肽(GSH)含量等进行分析。结果表明,干旱胁迫第7天,中花-11仍有部分叶片保持绿色且坚挺,干旱胁迫第11天时整株叶片变黄,甚至有的叶片变枯,经过干旱胁迫后的表型观察发现,中花-11比UC-32的抗旱能力强;对水稻的含水量、ABA含量、TBARS含量、GSH含量进行分析,表明中花-11的抗旱性优于UC-32,说明DHF-CBS基因增强了水稻的抗旱能力。In order to explore the drought resistance mechanism of DHF-CBS gene regulation in polyploid Oryza sativa L.under drought stress,two varieties of Oryza sativa L.were selected,namely polyploid plant Zhonghua-11(DHF-CBS gene)and mutant plant UC-32(non expressing DHF-CBS gene),the GUS chemical staining results,tissue moisture content,abscisic acid(ABA)con-tent,thiobarbituric acid reactive substance(TBARS)content,glutathione(GSH)content,etc.of two types of Oryza sativa L.were analyzed.The results showed that on the 7th day of drought stress,some leaves of Zhonghua-11 remained green and firm.On the 11th day of drought stress,the entire plant’s leaves turned yellow,and some leaves even withered,after observing the phenotype under drought stress,it was found that Zhonghua-11 had stronger drought resistance than UC-32.Analysis of the water content,ABA con-tent,TBARS content,and GSH content of Oryza sativa L.showed that the drought resistance of Zhonghua-11 was better than that of UC-32,indicating that the DHF-CBS gene enhanced the drought resistance of Oryza sativa L..

关 键 词：多倍体 水稻(Oryza sativa L.) DHF-CBS基因 抗旱性 

分 类 号：S511[农业科学—作物学]