21份猫尾草种子形态特征及SSR遗传多样性评价  

Evaluation of Morphological Characteristics and SSR Genetic Diversity of 21 Accessions of Timothy Seeds

作　　者：傅俊士 南丽丽[1] 张泽龙 吴世文 陈孝善 FU Junshi;NAN Lili;ZHANG Zelong;WU Shiwen;CHEN Xiaoshan(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Education Ministry/Key Laboratory of Forage Gerplasm Innovation and New Variety Breeding of Ministry of Agriculture and Rural Affairs,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/农业农村部饲草种质创新与新品种选育重点实验室,甘肃兰州730070

出　　处：《中国草地学报》2024年第9期23-33,共11页Chinese Journal of Grassland

基　　金：国家现代农业产业技术体系(CARS-34)。

摘　　要：为探究猫尾草(Phleum pratense)种质资源亲缘关系及种子表型差异,本研究采用形态特征测定并结合SSR分子标记对21份国内外猫尾草种质进行研究。结果表明,供试猫尾草种质种子面积、周长、长宽比、长度、宽度和千粒重间存在显著差异。10对SSR分子标记引物共扩增出144条带,多态性条带132条,多态性比率为88.74%。有效等位基因数(Ne)、Nei’s多样性指数(H)和Shannon指数(I)均值分别为1.6266、0.3507和0.5144。材料10676、7629和9038在种子形态聚类和SSR分子聚类时均属于第一类;材料10027和7019属于第二类,且5份材料种子与其他材料相比具有较大较圆、重量较重等优势,两类间遗传相似系数较小,遗传距离较大。本研究筛选出的种质可在新品种选育时作为优势亲本,以增加后代的遗传重组和提高杂种优势。In this research,morphological markers combined with SSR molecular marker were applied to investigate the kinship relationship of germplasm resources and seed phenotypic differences of Timothy(Phleum pratense)from 21 germplasms domestically and foreign.Results of this research demonstrated that there are significant differences between Timothy germplasms on seed area,perimeter,aspect ratio,length,width,and thousand seed weight.144 bands were amplified from 10 pairs of SSR molecular marker primers,in which contained 132 polymorphic bands,resulting a polymorphism ratio of 88.74%.Means of effective number of alleles(Ne),Nei's diversity index(H),and Shannon index(I)were 1.6266,0.3507,and 0.5144,respectively.Material No.10676,7629,and 9038 were clustered as groupⅠfor both seed morphology and SSR molecular.Material No.10027 and 7019 were clustered as groupⅡand the seeds of the five materials were characterized as larger,rounder,heavier compared to other materials.Meanwhile,the genetic coefficient of two cluster groups was relatively small,whereas the genetic distance was high.Germplasm selected from this research can be used as advantage parent in further new cultivar breeding for the aspects of recombination booster and heterosis improvement.

关 键 词：猫尾草 种子形态特征 SSR 遗传多样性 

分 类 号：S543.1[农业科学—作物学]