大豆GmEXPB3和GmEXPB6生物信息学分析及干旱和盐胁迫诱导表达  

Bioinformatics Analysis of GmEXPB3 and GmEXPB6 and Their Expression Induced by Drought and Salt Stress in Soybean

作　　者：李佳欣 孙莹莹 邓雅文 轩称心 马志强 马洪宇 张军 翟莹[1] LI Jiaxin;SUN Yingying;DENG Yawen;XUAN Chenxin;MA Zhiqiang;MA Hongyu;ZHANG Jun;ZHAI Ying(College of Life Science and Agro-Forestry of Qiqihar University,Qiqihar 161006,China;Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161005,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005

出　　处：《大豆科学》2024年第5期559-564,共6页Soybean Science

基　　金：黑龙江省大学生创新创业训练计划(S202310232113);黑龙江省省属高等学校基本科研业务费科研项目(145109506)。

摘　　要：膨胀素(Expansin,EXP)通过调控细胞壁的松弛在植物应对环境胁迫过程中起着重要作用。为研究EXP基因在大豆应对干旱和盐胁迫过程中的作用,本研究对大豆中的两个EXP基因(GmEXPB3和GmEXPB6)进行生物信息学分析,通过实时荧光定量RT-PCR(qRT-PCR)检测基因干旱和盐胁迫后的表达量。结果表明:GmEXPB3和GmEXPB6基因分别位于大豆第3和11号染色体上,编码的蛋白序列长度分别为267和277个氨基酸。GmEXPB3蛋白分子量为28.81 kDa,理论等电点为5.62;GmEXPB6蛋白分子量为29.37 kDa,理论等电点为4.95。GmEXPB3和GmEXPB6均为不稳定的亲水蛋白且定位于细胞壁中。GmEXPB3和GmEXPB6蛋白均含有1个保守的DPBB_1结构域。GmEXPB3蛋白与小麦TaEXPB10蛋白的亲缘关系最近,GmEXPB6蛋白与GmEXPB2蛋白亲缘关系最近。干旱胁迫能够诱导GmEXPB3的表达,但不能诱导GmEXPB6的表达。盐胁迫能够诱导GmEXPB3和GmEXPB6的表达。GmEXPB3的启动子区域含有5种与逆境有关的顺式作用元件,GmEXPB6的启动子区域含有4种与逆境有关的顺式作用元件。研究结果可为大豆EXPB基因抗逆机制的研究及应用提供理论依据。Expansin(EXP)plays an important role in plant response to environmental stress by regulating cell wall relaxation.To explore the role of EXP genes in soybean response to drought and salt stress,two EXP genes(GmEXPB3 and GmEXPB6)in soybean were analyzed by bioinformatics,and their expression levels were analyzed by real-time fluorescent quantitative PCR(qRT-PCR).The GmEXPB 3 and GmEXPB6 were located on chromosomes 3 and 11 of soybean,respectively,and encoded proteins containing 267 and 277 amino acids,respectively.The molecular weight of GmEXPB3 protein is 28.81 kDa and the theoretical isoelectric point is 5.62.The molecular weight of GmEXPB6 protein is 29.37 kDa and the theoretical isoelectric point is 4.95.GmEXPB3 and GmEXPB6 are both unstable hydrophilic proteins and localized in the cell wall.Both GmEXPB3 and GmEXPB6 proteins contained a conserved DPBB_1 domain.GmEXPB3 protein was closely related to TaEXPB10 protein in wheat,and GmEXPB6 protein was closely related to GmEXPB2 protein in soybean.Drought stress could induce the expression of GmEXPB 3,but not GmEXPB6.Salt stress could induce the expression of GmEXPB 3 and GmEXPB6.The promoter region of GmEXPB 3 contained five stress related cis-elements.The promoter region of GmEXPB6 contained four stress related cis-elements.The results provide a theoretical basis for the study and application of stress-resistance mechanism of EXPB gene in soybean.

关 键 词：大豆 膨胀素 EXPB 生物信息学分析 非生物胁迫 表达分析 

分 类 号：S565.1[农业科学—作物学]