一株红树林来源施氏假单胞菌的分离及其在水质净化中的应用  

Isolation of a mangrove source of Pseudomonas stelleri and its application in water purification

作　　者：吴雯雯 谢梓欣 喻文婷 姚玉梅 肖淑滢 马军 WU Wenwen;XIE Zixin;YU Wenting;YAO Yumei;XIAO Shuying;MA Jun(Hainan Tropical Ocean University,Hainan Provincial Key Laboratory of Conservation and Utilization of Tropical Marine Fishery Resources,Hainan 572022,Hainan China)

机构地区：[1]海南热带海洋学院,海南省热带海洋渔业资源保护与利用重点实验室,海南海口572022

出　　处：《黑龙江水产》2024年第5期521-528,共8页Northern Chinese Fisheries

基　　金：海南高校大学生创新创业计划项目“固定化微生物在养殖废水中的应用研究(编号:202311100010)”。

摘　　要：随着水产养殖业的快速发展,养殖水环境日趋恶化,养殖动物病害频发。益生菌因其对环境友好和安全而被广泛应用于养殖水环境的改善中。但细菌易受外界环境影响,稳定性不高,这严重制约了其在养殖水环境中的应用。固定化微生物技术可以大幅提高优势菌种在水环境中的稳定性,为细菌在水质净化应用提供了方向。该研究从三亚白鹭公园红树林根际土壤中筛选出一株高降解亚硝酸盐的菌株X1,该菌株在第4 d时亚硝酸盐降解率可达到97.18%。经16S rRNA基因的系统发育树分析表明,该菌株属于施氏假单胞菌(Pseudomonas stutzeri)。将该菌株与海藻酸钠-壳聚糖复合载体结合形成固定化小球,投入到养殖废水中,亚硝酸盐降解率在第2天就可以达到98.15%,第3天和第4天降解率保持平稳,并无亚硝酸盐积累,在第5天达到98.35%。该研究结果证实了施氏假单胞菌能够高效、快速且安全地降解水体中的亚硝酸盐,使用固定化技术能够更好地提高该菌株的降解速率及其稳定性,这为固定化施氏假单胞菌应用于水体净化提供了一定的参考。With the rapid development of the aquaculture industry,the aquaculture water environment is deteriorating day by day,and the diseases of farmed animals are frequent.Aquatic probiotics are widely used in the improvement of aquaculture water environment because of their environmental friendliness and safety.However,bacteria are susceptible to the external environment and have low stability,which seriously restricts their application in the aquaculture water environment.The immobilized microbial technology can greatly improve the stability of dominant bacteria in the water environment,which provides a direction for the application of bacteria in water purification.In this study,a highly nitrite-degrading strain X1 was screened from the rhizosphere soil of mangrove forest in Egret Park,Sanya,and the nitrite degradation rate of this strain could reach 97.18%on the 4th day.Phylogenetic tree analysis of 16S rRNA gene showed that the strain belonged to Pseudomonas stutzeri.The strain was combined with sodium alginate-chitosan composite carrier to form immobilized pellets,which were put into aquaculture wastewater,and the nitrite degradation rate could reach 98.15%on the 2nd day,and the degradation rate on the 3rd and 4th days remained stable,and there was no nitrite accumulation,reaching 98.35%on the 5th day.The results confirmed that Pseudomonas stutzeri schudei could degrade nitrite in water efficiently,quickly and safely,and the immobilization technology could better improve the degradation rate and stability of the strain,which provided a certain reference for the application of immobilized Pseudomonas stutzeri in water purification.

关 键 词：红树林 施氏假单胞菌(Pseudomonas stutzeri) 亚硝酸盐 水产养殖 固定化 

分 类 号：S9[农业科学—水产科学]