多巴胺受体在小肠的分布与定位  

Distribution and localization of dopamine receptor in small intestines

作　　者：陈钧钧 周立[1] 苏田 王现伟 张海龙 王志勇[1] CHEN Jun-jun;ZHOU Li;SU Tian;WANG Xian-wei;ZHANG Hai-long;WANG Zhi-yong(Department of Human Anatomy and Histoembryology,Xinxiang Medical University,He'nan Xinxiang 453003,China;He'nan Key Laboratory of Medical Tissue Regeneration,He'nan Xinxiang 453003,China;School of Basic Medicine,He'nan University,He'nan Kaifeng 475000,China)

机构地区：[1]新乡医学院人体解剖学与组织学胚胎学系,河南新乡453003 [2]河南省医用组织再生重点实验室,河南新乡453003 [3]河南大学基础医学院,河南开封475000

出　　处：《解剖学报》2024年第5期612-618,共7页Acta Anatomica Sinica

基　　金：河南省科技发展计划项目([2020]12号202102310348);新乡医学院博士科研启动基金(300505276);河南省高等学校青年骨干教师培养计划(2017GGJS107)。

摘　　要：目的探讨多巴胺受体D3~D5在不同物种小肠的分布和定位。方法应用免疫组织化学和免疫印迹法,分别检测D3~D5在小鼠、大鼠和恒河猴小肠的分布及表达水平,通过免疫荧光双标技术观察小肠固有层(LP)免疫球蛋白A阳性浆细胞(IgA^(+)PC)中D3~D5的表达。结果D3和D5在小鼠、大鼠和恒河猴小肠的上皮层、LP、黏膜下神经丛(SMP)和肌间神经丛(MP)内广泛分布;D4在小鼠和恒河猴小肠的分布与D3和D5一致,但在大鼠小肠仅分布于上皮层和LP。小鼠和大鼠LP内的IgA^(+)PC均表达D3和D5,不表达D4。结论D3和D5在小鼠、大鼠和恒河猴小肠具有相似的分布和定位模式,D4则存在物种差异。多巴胺可能参与调节IgA^(+)PC的功能。Objective To explore the distribution and localization of dopamine receptor D3-D5 in the small intestine of different species.Methods The distribution and expression of D3-D5 in the small intestine of mice,rats and rhesus monkeys were detected by immunohistochemistry and Western blotting.The expression of D3-D5 in immunoglobulin A positive plasma cells(IgA^(+)PC)located in the lamina propria(LP)were detected by immunofluorescence double labeling.Results D3 and D5 were widely distributed in the epithelium,LP,submucosal plexus(SMP)and intermuscular plexus(MP)of the small intestine in mice,rats and rhesus monkeys.The distribution of D4 in the small intestinal of mice and rhesus monkeys were consistent with the result of D3 and D5.D4 was distributed only within the epithelium and LP of rat smallintestine.D3 and D5 were expressed in the IgA^(+)PC in the LP of mice and rats,whereas D4 was not.Conclusion The distribution and localization pattern of D3 and D5 are similar in the small intestine of mice,rats and rhesus monkeys,whereas those of D4 vary between different species.Dopamine may be involved in regulating the functions of IgA^(+)PC.

关 键 词：多巴胺受体 小肠 免疫球蛋白A阳性浆细胞 免疫印迹法 免疫荧光 小鼠 大鼠 恒河猴 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]