避瘟解毒颗粒对LPS诱导急性肺损伤小鼠保护作用的研究  

Protective Effect of BiWen Jiedu Granules on LPS-induced Acute Lung Injury in Mice

作　　者：王园 杨允 王博[2] 崔妮 王海峰 张红[1] 刘洋[1] 龙凯花 卫向锋 WANG Yuan;YANG Yun;WANG Bo;CUI Ni;WANG Haifeng;ZHANG Hong;LIU Yang;LONG Kaihua;WEI Xiangfeng(Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710003,China;Northwest University,Xi'an 710069,China;Xi'an TCM Hospital of Encephalopathy,Xi'an 710032,China;Xi'an TCM Preparation Engineering Technology Research center,Xi'an 710032,China;Shaanxi Provincial Armed Police Corps Hospital,Xi'an 710000,China)

机构地区：[1]陕西省中医药研究院,陕西西安710003 [2]西北大学,陕西西安710069 [3]西安中医脑病医院,陕西西安710032 [4]西安市中药制剂工程技术研究中心,陕西西安710032 [5]武警陕西省总队医院,陕西西安710000

出　　处：《特产研究》2024年第5期42-47,共6页Special Wild Economic Animal and Plant Research

基　　金：陕西省重点研发计划项目(2022ZDXM-SF-06);秦创原中医药创新研发转化项目(2022-QCYZH-006);“秦药”研发重点实验室(2021-QYPT-001);“秦药”质量提升工程项目(2021-QYZL-01);陕西省科协青年人才托举计划项目(20230321);西安市科技计划项目(23YXYJ0142);陕西省科技厅一般项目(2024SF-YBXM-489)。

摘　　要：探究避瘟解毒颗粒对脂多糖(LPS)致急性肺损伤(ALI)小鼠的保护作用。将30只C57小鼠随机分为正常组、模型组、地塞米松组、避瘟解毒颗粒高、低剂量组,每组6只,每天给药1次,连续给药7 d。模型组、地塞米松组、避瘟解毒颗粒给药组气管滴注脂多糖(LPS)构建小鼠急性肺损伤模型(ALI)。造模后72 h取材检测,分析小鼠体重变化情况;血常规指标;胸腺指数;ELISA法检测肺泡灌洗液(BALF)中IL-1β、IL-6、TNF-α水平;HE染色法观察肺组织病理变化。与模型组比较,避瘟解毒颗粒高剂量组小鼠血清中血常规指标,BALF中炎性细胞因子IL-6、TNF-α含量,肺组织损伤程度均有不同程度的降低(P<0.05,P<0.01),且高剂量组能显著升高ALI小鼠的胸腺指数(P<0.01)。避瘟解毒颗粒对LPS诱导急性肺损伤发挥一定的保护作用,改善小鼠肺组织的病理改变,增强免疫功能,其作用机制可能与抑制促炎细胞因子的分泌及发挥抗炎作用有关。To explore the effect BiWen Jiedu Granules on LPS-induced acute lung injury in mice.Thirty male C57 mice were randomly divided into the normal group,the model group,the dexamethasone group,and the low-dose and high-dose groups of BiWen Jiedu Granules,with 6 in each group,which were administered once a day for 7 days.The acute lung injury model was established by tracheal infusion of lipopolysaccharide(LPS)in the model group,the dexamethasone group,and the low-dose and high-dose groups of BiWen Jiedu Granules group.The material was taken at 72 h,the rats had their body weights,blood routine detected,their bronchoalveolar lavage fluid(BALF)levels of IL-1,IL-6 and TNF-detected by ELISA;the lung histopathological changes were observed by the HE staining.Compared with the modeld group,the the high-dose BiWen Jiedu Granules group shared lower degree of blood routine,levels of inflammatory cytokine IL-6 and TNF-in BALF,lung injury(P<0.05,P<0.01),high-dose groups can increase the thymus index of ALI mice obviously(P<0.01).BiWen Jiedu Granules plays a protective role in LPS-induced acute lung injury in mice,improve the histopathological changes of lung,enhance immune function and its mechanism may be related to inhibiting the secretion of inflammatory cytokines function.

关 键 词：避瘟解毒颗粒 脂多糖 急性肺损伤 炎症因子 

分 类 号：R285.5[医药卫生—中药学]