具有力学与结构稳定性多组分纤维肌腱防粘连膜制备及性能  

Preparation and performance of multi-component fiber tendon anti-adhesion membrane with mechanical and structural stability

作　　者：汪孟姣 吴新康 王富军[1,2] 王璐[1,2] 黄超 李超婧 WANG Meng-jiao;WU Xin-kang;WANG Fu-jun;WANG Lu;HUANG Chao;LI Chao-jing(Key Laboratory of Textile Science&Technology,Ministry of Education,Donghua University,Shanghai201620,China;College of Textiles,Donghua University,Shanghai201620,China;Shanghai Haohai Biological Technology Co.Ltd.,Shanghai201103,China)

机构地区：[1]东华大学纺织面料技术教育部重点实验室,上海201620 [2]东华大学纺织学院,上海201620 [3]上海昊海生物科技股份有限公司,上海201103

出　　处：《生物医学工程与临床》2024年第5期602-610,共9页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金青年科学基金项目(32201080);上海市浦江人才计划项目(21PJ1420800);上海市“科技创新行动计划”生物医药科技支撑项目(21S31900700);111计划2.0(BP0719035)。

摘　　要：目的引入疏水聚己内酯(PCL),改善壳聚糖(CS)/聚乙烯醇(PVA)混纺膜的性能,研究制备性能优良的肌腱防粘连材料的可行性。方法实验以具有低蛋白吸附性的PVA、成纤维细胞增殖抑制作用的CS和疏水性聚合物PCL为原料,通过共轭静电纺丝技术制备不同组分(CS∶PCL∶PVA=3∶5∶2、3∶4∶3、3∶3∶4、3∶2∶5)的纤维膜(CS/PCL/PVA膜,简称CPA膜)。采用傅里叶红外光谱仪分析CPA膜组成,扫描电子显微镜、万能实验仪及模拟体液预处理的方式探究了PVA和PCL含量对形貌、力学、结构稳定性的影响,进一步使用热重分析仪及细胞实验分析CPA膜热性能、细胞毒性和黏附性。结果CPA膜红外谱图中同时出现CS、PCL及PVA特征峰,且未产生新的基团或化学键。根据体液稳定性与微观形貌测试结果可知,PCL的引入一方面降低了CPA膜的纤维平均直径;与CS/PVA膜相比,CPA膜(CS∶PCL∶PVA=3∶5∶2)的断裂强度和伸长率分别提高了约60%和40%,弹性模量相当且与文献数据一致。另一方面改善CPA膜湿环境下力学性能失效和卷曲皱缩的问题。热重分析结果显示多组分纤维膜分解温度在250℃~600℃,而实际应用环境温度显著低于热分解温度,具有热稳定性,即防粘连膜不会因温度而导致结构性能的损失。细胞实验结果显示,CPA膜(CS∶PCL∶PVA=3∶5∶2)具有良好的细胞相容性及一定的体外抗细胞黏附性。第3天CA和CPA纤维膜的细胞存活率分别为(96.533±0.341)%、(101.877±11.001)%(P>0.05)。培养7 d后,CPA光密度值为0.223±0.002,显著低于CA(0.292±0.007)。结论CPA膜具有增强的力学强度与湿态稳定性,更适应肌腱部位的受力环境。表现出更优异的抗细胞黏附效果,CS∶PCL∶PVA的质量比为3∶5∶2为最优参数的防粘连膜材料。具有肌腱术后防粘连领域的应用潜力。Objective To study the feasibility of preparing tendon anti adhesion materials with excellent performance,with introducing of hydrophobic poly(caprolactone)(PCL)to improve the performance of chitosan(CS)/polyvinyl alcohol(PVA)blended films.Methods The raw materials(CS/PCL/PVA membranes,abbreviation as CPA membranes)were polyvinyl alcohol(PVA)with low protein adsorption,chitosan(CS)with fibroblast proliferation inhibition and hydrophobic polymer polycaprolactone(PCL)(CS∶PCL∶PVA=3:5:2,3:4:3,3:3:4,3:2:5).The composition of the anti-adhesion membrane was further characterized by Fourier transform infrared spectroscopy.The fiber membranes with different components were prepared by conjugate electrospinning technique.The effects of PVA and PCL content on morphology,mechanical and structural stability were investigated by scanning electron microscopy,universal tester and simulated body fluid pretreatment.Thermal properties,cytotoxicity and adhesion of the anti-adhesion membrane were further characterized by thermogravimetric analyzer and cell experiments.Results The infrared analysis results showed that there was no new characteristic peak in CS/PCL/PVA membrane,indicating that preparation of fiber membrane did not generate any new functional groups or chemical bonds.The results of body fluid stability and microstructure test results showed that the introduction of PCL reduced fiber mean diameter of CPA membrane,and improved mechanical properties failure and crimp shrinkage of CS/PVA membrane in wet environment.Compared with CS/PVA membranes,the fracture strength and elongation of CPA membranes(CS:PCL:PVA=3:5:2)were increased by about 60%and 40%,respectively.The elastic modulus was comparable and consistent with the literature data.On the other hand,it improved the mechanical performance failure and solved curling and wrinkling problems of CPA films in wet environments.The results of thermogravimetric analysis results showed that the decomposition temperature of multi-component fiber membrane was 250℃-600℃,and a

关 键 词：防粘连屏障 静电纺丝 纤维膜 肌腱 湿态稳定性 

分 类 号：R318.08[医药卫生—生物医学工程] TS101.4[医药卫生—基础医学]