河北邯郸和北京杨树的叶锈病菌及检疫性物种Melampsora medusae的监测  

Poplar leaf rust fungi from Hebei’s Handan and Beijing and detection of quarantine species Melampsora medusae

作　　者：胥卓尔 廖伊倩 吕友红 王有方 秦志林 段维军 朱小琼 XU Zhuoer;LIAO Yiqian;LU Youhong;WANG Youfang;QIN Zhilin;DUAN Weijun;ZHU Xiaoqiong(Key Lab of Pest Monitoring and Green Management,Ministry of Agriculture and Rural Affairs/Department of Plant Pathology,College of Plant Protection,China Agricultural University,Beijing 100193,China;Ningbo Academy of Inspection and Quarantine,Ningbo 315012,Zhejiang,China;Feixiang District Forestry Bureau,Handan 057550,Hebei,China;Ningbo Customs District P.R.China,Ningbo 315012,Zhejiang,China)

机构地区：[1]中国农业大学植物保护学院植物病理学系农业农村部作物有害生物监测与绿色防控重点实验室,北京100193 [2]宁波检验检疫科学技术研究院,浙江宁波315012 [3]邯郸市肥乡区林业局,河北邯郸057550 [4]宁波海关,浙江宁波315012

出　　处：《菌物学报》2024年第9期36-44,共9页Mycosystema

基　　金：农业农村部科技教育司课题(13230107);山西省农业农村厅课题(ZHZB20230061);中国农业大学2022年本科教学改革项目;中国农业大学2020年研究生教学改革项目;中国农业大学植物保护学院2021年本科教学改革项目。

摘　　要：杨树广泛应用于木材生产、防护林和园林景观建造,具有显著的经济价值和生态价值。杨树叶锈病主要为害杨树叶片,幼苗叶片更容易受害,2023年河北邯郸杨树苗圃叶锈病发生严重危害。本研究针对河北邯郸及北京发生的杨树叶锈病病菌,通过观察夏孢子形态特征并结合rDNA ITS序列差异进行鉴定。结果发现,采自两地的杨树叶锈病菌均为松杨栅锈菌Melampsora larici-populina,未发现我国对外检疫杨树叶锈病菌M.medusae。本研究详细比较了杨树叶锈病菌的ITS序列差异,可以将M.medusae与Melampsora属中其他种区分开,为后续杨树叶锈病病原的鉴定和监测提供了基础数据。Poplars are widely used in wood production,shelterbelt,and garden landscape construction,with significant economic and ecological value.Poplar leaf rust mainly occurs on poplar leaves with a severe impact on seedling growth.In 2023,leaf rust occurred seriously in the poplar nursery in Handan of Hebei Province.In this study,the pathogen of poplar leaf rust from Beijing and Hebei was identified by observing morphological characteristics of urediniospores on leaves and sequence comparison of rDNA ITS regions.The results showed that the pathogen responsible for poplar leaf rust in Beijing and Hebei was Melampsora larici-populina.The quarantine species M.medusae was not detected in these test samples.Eight distinct loci of the ITS sequence were used to differentiate M.medusae from the other closely related Melampsora species,providing some data for monitoring the quarantine species M.medusae.

关 键 词：杨树叶锈病 松杨栅锈菌 种类鉴定 ITS序列 

分 类 号：S763.7[农业科学—森林保护学]