进境比利时杜鹃中杜鹃塞弗特菌的检疫鉴定  

Quarantine identification of Seifertia azaleae in rhododendron imported from Belgium

作　　者：龚静如 田沂民 徐飞 虞天华 周淑辉 易建平 GONG Jingru;TIAN Yimin;XU Fei;YU Tianhua;ZHOU Shuhui;YI Jianping(Technical Center for Animal,Plant and Food Inspection and Quarantine of Shanghai Customs,Shanghai 200135,China)

机构地区：[1]上海海关动植物与食品检验检疫技术中心,上海200135

出　　处：《菌物学报》2024年第9期45-51,共7页Mycosystema

摘　　要：从进境比利时酒红杜鹃种苗花芽上获得1株真菌分离物8033-2,通过形态学特征比较、内部转录间隔区(ITS)、翻译伸长因子(EF-1α)以及RNA聚合酶Ⅱ第二大亚基(RPB)序列分析和致病性测定方法对分离物进行了鉴定。形态学观察结果表明,分离物8033-2初期菌落白色,茸毛状,气生菌丝较多;后期菌落致密,正面深灰色,背面橄榄绿色。分生孢子单细胞,平滑,近球形或椭圆形,灰褐色,大小为8.04(6.32-11.49)×5.60(4.38-7.40)μm。其ITS、EF-1α、RPB序列与GenBank中多株杜鹃塞弗特菌相应序列的相似性分别为100%、100%和99.90%;基于ITS、EF-1α和RPB序列的系统发育树显示,分离物8033-2与杜鹃塞弗特菌处于同一分支。人工伤口接种健康杜鹃花芽引起褐变枯死症状。根据上述结果,将分离物8033-2鉴定为杜鹃塞弗特菌Seifertia azaleae,这是我国口岸首次截获该种检疫性有害生物。A fungal strain 8033-2 isolated in bud of rhododendron imported from Belgium was identified by morphological characteristics,sequence analysis of internal transcribed spacer(ITS)region,translation elongation factor(EF-1α)and RNA polymerase Ⅱ second largest subunit(RPB)gene and pathogenicity test.The colony of isolate 8033-2 is white,initially hairy,with more aerial hyphae,and turned into dense,grey,olive green when well developed and mature.Conidia single-celled,smooth,nearly spherical or elliptical,gray brown,with a size of 8.04(6.32-11.49)×5.60(4.38-7.40)μm.The sequence similarities of ITS,EF-1α and RPB genes between isolate 8033-2 and the corresponding sequences of Seifertia azaleae in GenBank were 100%,100% and 99.90%,respectively.The phylogenetic tree based on ITS,EF-1α and RPB gene sequence showed that isolate 8033-2 clustered with S.azaleae.Artificial wound inoculation caused typical symptom of brown necrosis in healthy buds.The isolate 8033-2 was identified as Seifertia azaleae,which is the first interception of this quarantine pest at border ports of China.

关 键 词：杜鹃塞弗特菌 杜鹃 鉴定 

分 类 号：S41[农业科学—植物保护]