内源性APE1辅助的DNA纳米探针用于miR-21的原位成像检测  

Endogenous APE1 Assisted DNA Nanoprobe for in Situ Imaging Detection of miR-21

作　　者：苏聪 李英 Su Cong;Li Ying(College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266045,China)

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266045

出　　处：《山东化工》2024年第17期173-176,180,共5页Shandong Chemical Industry

基　　金：国家自然科学基金(21775080)。

摘　　要：以miRNA作为靶标对肿瘤细胞进行原位成像有利于提高肿瘤疾病诊断和检测的准确性。然而,受限于细胞内miRNA的低丰度以及胞内复杂的生理环境,传统核酸探针一对一信号输出模式的检测灵敏度不高且易于造成假阳性信号,这使得其在原位检测方面的性能大打折扣。对此,开发了一种以金纳米粒子为载体,miR-21和APE1共同激活的walker型DNA纳米探针,并成功实现了针对肿瘤细胞的高灵敏、特异性原位成像,且有效降低了假阳性信号。It is beneficial for improving the accuracy of tumor disease diagnosis and detection by using miRNA as a target for in situ imaging of tumor cells.However,limited by the low abundance of intracellular miRNA and the complex physiological environment within cells,the detection sensitivity of traditional nucleic acid probes'one-to-one signal output mode is low and prone to false positive signals,significantly compromising the performance in in situ detection.Thus,a walker-based DNA nanoprobe activated by miR-21 and APE1 was developed using gold nanoparticles as a carrier.By using this DNA nanoprobe,high sensitivity and specificity in situ imaging of tumor cells was successfully achieved,and false positive signals were effectively reduced.

关 键 词：microRNA APE1 金纳米粒子 WALKER 原位成像 

分 类 号：O657.1[理学—分析化学]