茄子绿果基因的精细定位及其KASP标记开发  

Fine-Mapping and KASP Marker Development for the Gene Underlying Green Fruit in Eggplant

作　　者：夏志磊 俞冰昕 杨梦 连子林 官利兰 何艺超 颜爽爽 曹必好[1] 邱正坤 XIA Zhilei;YU Bingxin;YANG Meng;LIAN Zilin;GUAN Lilan;HE Yichao;YAN Shuangshuang;CAO Bihao;QIU Zhengkun(Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,College of Horticulture,South China Agricultural University,Guangzhou 510642,China;Guangdong Enping Agricultural Technology Extension Center,Jiangmen,Guangdong 529499,China)

机构地区：[1]华南农业大学园艺学院,农业农村部华南地区园艺作物生物学与种质创制重点实验室,广州510642 [2]广东省恩平市农业技术推广服务中心,广东江门529499

出　　处：《园艺学报》2024年第9期2008-2018,共11页Acta Horticulturae Sinica

基　　金：广东省基础与应用基础研究基金项目(2023A1515030070,2022A1515010734);广东省重点研发计划项目(2022B020208003);广东省种业振兴项目(2022-NPY-00-026);广东省现代农业产业技术果菜产业体系创新团队项目(2023KJ110);海南省重点研发项目(ZDYF2023XDNY041)。

摘　　要：以白茄高代自交系E802与绿茄高代自交系NY为亲本构建的F_(2)群体为试验材料,对茄子果色基因进行遗传连锁分析。结果显示,在304株F_(2)群体中绿茄与白茄单株数符合3︰1孟德尔单基因分离比,其F1植株果实为绿色,表明绿茄对白茄为显性,受一个主效基因控制。以F_(2)及其F_(2:3)家系为材料,采用BSA-seq结合KASP分子标记连锁分析,将绿茄基因精细定位于8号染色体末端约683.4kb的范围内,其遗传距离约为0.17cM。根据茄子基因组注释信息,该区间内含有46个基因。结合序列分析、同源分析将SmAPRR2作为绿茄关键候选基因。SmAPRR2在白茄E802中存在5.27kb的大片段缺失,使其缺失第7~11号外显子,导致其功能缺失。进一步开发了与SmAPRR2紧密连锁的KASP分子标记22QP296,其在F_(2)分离群体中的基因型和表型一致率为100%,在194份茄子自然材料中基因型和表型一致率为94.85%,可作为茄子果色分子育种的分子标记。An F2 population developing by a white fruit eggplant line E802 and a green fruit eggplant line NY was used for the genetic analysis of the gene underlying green fruit color.The segregation ratio of green fruit and white in the 304 individuals in the F2 population was 3︰1,indicating that the green phenotype of NY is controlled by a single dominant locus.Combing with the BSA-seq and linkage-based QTL mapping,the gene underlying green fruit color of eggplant was fine-mapped into 683.4 kb interval on chromosome 8 using F2 and the F2:3 population.The genetic distance of this locus is 0.17 cM.Among the 46 genes in the interval,SmAPRR2 was the best candidate gene.The 5.27 kb deletion was detected in the E802,resulting the SmAPRR2 lacking the exon 7 to 11.In addition,a tightly linked to SmAPRR2 KASP marker,22QP296,was development.The accuracies of 22QP296 for predicting the skin color(green or white)in the F2 population and the 194 cultivars were 100%and 94.85%,respectively,indicating that this KASP marker can be used in molecular marker-assisted selection in eggplant breeding.

关 键 词：茄子 果实颜色 基因定位 KASP标记 绿果基因 APRR2 

分 类 号：S641.1[农业科学—蔬菜学]