分子标记辅助构建甜瓜矮化基因Cmerecta近等基因系  

Molecular Markers Assisted Construction of Cmerecta Near-Isogenic Line of Melon Dwarfing Gene

作　　者：刘港运 段世享 许娜娜 郭姚淼 豆峻岭 杨森 牛欢欢 刘东明 杨路明[1] 胡建斌[1] 朱华玉 LIU Gangyun;DUAN Shixiang;XU Nana;GUO Yaomiao;DOU Junling;YANG Sen;NIU Huanhuan;LIU Dongming;YANG Luming;HU Jianbin;ZHU Huayu(College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学园艺学院,郑州450002

出　　处：《园艺学报》2024年第9期2048-2062,共15页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(32072600,31872133,32172574);河南省高校科技创新人才支持计划项目(23HASTIT034);河南省优秀青年科学基金项目(222300420050)。

摘　　要：前期通过图位克隆的方法精细定位到一个控制节间长度的CmERECTA基因,并开发了一个和甜瓜矮化共分离的标记Cmerecta-dCAPS2。为了进一步研究其功能,以甜瓜自交系TopMark为轮回亲本,矮化自交系M406为供体亲本,利用Cmerecta-dCAPS2为前景选择标记,对筛选出的102对在亲本间多态性好、染色体上分布相对均匀的SSR标记为背景选择标记,在TopMark遗传背景下构建Cmerecta近等基因系,并统计分析其表型。结果显示,BC1F1和BC2F1世代最高背景回复率单株的背景回复率分别为80.61%、98.57%。背景回复率最高的3个BC2F2代Cmerecta纯系植株的表型显示其株高均为矮化,叶片变圆。该近等基因系可作为后续研究甜瓜矮化基因CmERECTA的调控机理以及应用的基础材料。In the early stage,a CmERECTA gene that controls internode length was finely mapped by map cloning,and a marker Cmerecta-dCAPS2 co-separated from melon dwarf was developed.In order to further study its function,the 102 pairs of SSR markers with good polymorphism and relatively uniform distribution on chromosomes were detected by using the melon inbred line TopMark as the reincarnation parent,the dwarf inbred line M406 as the donor parent,and the Cmerecta-dCAPS2 as the foreground selection marker,the background recovery rate of the SSR markers with good polymorphism and relatively uniform distribution on the chromosomes was detected,and the Cmerecta near-isogenic line was constructed under the TopMark genetic background,and its phenotype was statistically analyzed.The results showed that the highest background response rates per plant of BC1F1 and BC2F1 generations were 80.61%and 98.57%,respectively.The phenotypes of the three BC2F2 generation Cmerecta pure lines with the highest background recovery rate showed that the plant height was dwarf and the leaves were rounded.The construction of this near-isogenic line can be used as a basic material for subsequent research on the regulatory mechanism and application of CmERECTA,a dwarf gene in melon.

关 键 词：甜瓜 分子标记 近等基因系 矮化基因 CmERECTA 辅助育种 

分 类 号：S652[农业科学—果树学]