三褶虾脊兰花发育相关基因RT-qPCR内参基因的筛选  

Selection and Validation of Reference Genes for RT-qPCR of Flower Development in Calanthe triplicata

作　　者：吴悠 冀宏宇 邓淑雯 梁翠怡 万志庭 吴沙沙[1] 翟俊文[1] WU You;JI Hongyu;DENG Shuwen;LIANG Cuiyi;WAN Zhiting;WU Shasha;ZHAI Junwen(Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,College of Landscape Architecture and Art,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]福建农林大学风景园林与艺术学院,兰科植物保护与利用国家林业和草原局重点实验室,福州350002 [2]北京林业大学园林学院,北京100083

出　　处：《园艺学报》2024年第9期2063-2074,共12页Acta Horticulturae Sinica

基　　金：兰科植物保育与利用研究中心建设和管理项目(KH230350A);植物新资源开发与利用服务团队项目(11899170124B)。

摘　　要：为了推进三褶虾脊兰(Calanthe triplicata)花发育相关基因表达模式的研究,使用geNorm、NormFinder、BestKeeper和RefFinder等4种算法在整花、花部各结构和混合样品中鉴定和评估了9个候选内参基因。结果显示,尽管各处理之间内参基因的稳定性排名略有差异,但UBQ1和UBQ2表现最为稳定。通过RT-qPCR扩增了与花发育相关的4个靶基因,结果佐证了UBQ1和UBQ2是研究三褶虾脊兰花发育基因表达的最佳内参基因。To facilitate studies on gene expression in Calanthe triplicata,nine candidate reference genes were identified and evaluated across three different groups(the whole flower group,the floral structures group and the mixed group)using geNorm,NormFinder,BestKeeper and RefFinder.The results showed although there were slight differences in the ranking of RGs between groups,UBQ1 and UBQ2 were the most stable.To valid the accuracy of the evaluation results,four target genes related to flower development were amplified by RT-qPCR,the results further supported that UBQ1 and UBQ2 were the most optimal for reference genes.

关 键 词：三褶虾脊兰 RT-QPCR 内参基因 稳定性评价 

分 类 号：S682.31[农业科学—观赏园艺]