Unraveling the role of formate in improving nitrogen removal via coupled partial denitrification-anammox  

作　　者：Wanlu Zhu Rui Xiao Min Xu Wenbo Chai Wenlong Liu Zhengyu Jin David Ikumi Huijie Lu 

机构地区：[1]Key Laboratory of Environmental Remediation and Ecological Health(Ministry of Education),College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,China [2]China National Nuclear Corporation,Beijing Research Institute of Chemical Engineering and Metallurgy,Beijing 100029,China [3]College of Environment,Zhejiang University of Technology,Hangzhou 310014,China [4]Key Laboratory of Ecology and Environment in Minority Areas,National Ethnic Affairs Commission,College of Life&Environmental Sciences,Minzu University of China,Beijing 100081,China [5]Water Research Group,Department of Civil Engineering,University of Cape Town,Rondebosch 7700,South Africa [6]Key Laboratory of Water Pollution Control and Environmental Safety,Hangzhou 310058,China

出　　处：《Frontiers of Environmental Science & Engineering》2024年第9期79-90,共12页环境科学与工程前沿（英文）

基　　金：funded by the Zhejiang Provincial Natural Science Foundation of China(No.LR23B070002);the General Program of the National Natural Science Foundation of China(No.22276165).

摘　　要：The addition of traditional carbon sources(e.g.,acetate)could favor heterotrophic overgrowth in partial denitrification coupled with anammox(PD–A)systems,thus hindering the performance and stability of this novel wastewater nitrogen removal technology.Therefore,it is necessary to develop an effective,environmentally friendly,and inexpensive alternative.This study demonstrated the potential of formate to enhance the performance and community stability of PD–A under mainstream conditions.In a laboratory-scale biofilm reactor,formate addition(COD/NO_(3)^(–)–N=1.75)improved nitrogen removal efficiency(from 72.1±3.5%to 81.7±2.7%),EPS content(from 106.3±8.1 to 163.0±15.5 mg/gVSS)and increased anammox bacteria growth(predominantly Candidatus Brocadia,from 29.5±0.7%to 34.5±5.4%)while maintaining stable heterotrophs dominated by methylotrophic Desulfobacillus.FISH–NanoSIMS revealed a formate uptake using Ca.Brocadia and Desulfobacillus,with Ca.Brocadia being the major contributor to partial nitrate reduction to nitrite.Desulfobacillus can synthesize diverse hydrophobic amino acids and provide key nutrients for Ca.Brocadia.To achieve comparable nitrogen removal,the cost of the formate-driven PD–A process should be 11.2%lower than that of acetate.These results greatly enrich our understanding of C1 metabolism represented by formate in anammox communities and its application in the context of coupling partial denitrification–anammox toward enhanced nitrogen removal in global wastewater treatment systems.

关 键 词：FORMATE Mixotrophic growth Partial denitrification-anammox Metabolic interaction FISH-NanoSIMS 

分 类 号：X703[环境科学与工程—环境工程]