鱼源枯草芽孢杆菌J5发酵棉粕条件优化  

Optimization of the Fermentation Conditions for Cottonseed Meal by Fish-derived Bacillus subtilis J5

作　　者：徐一力 戴子凡 陈铭川 丁皓天 吴冰 纪逸 陈香凝 丁祝进 魏朝青 程汉良 孙宜防 许建和 XU Yili;DAI Zifan;CHEN Mingchuan;DING Haotian;WU Bing;JI Yi;CHEN Xiangning;DING Zhujin;WEI Chaoqing;CHENG Hanliang;SUN Yifang;XU Jianhe(School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Sun Yifang Aquaculture Professional Cooperative in Zhouji Township,Shuyang County,Suqian 223699,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏连云港222005 [2]沭阳县周集乡孙宜防水产养殖专业合作社,江苏宿迁223699

出　　处：《江苏海洋大学学报（自然科学版）》2024年第3期1-8,共8页Journal of Jiangsu Ocean University:Natural Science Edition

基　　金：江苏省科技支撑项目(苏北科技专项)(SZ-SQ2021034);江苏省自然科学基金资助项目(BK20201465);江苏省研究生科研与实践创新计划项目(SJCX22_1663)。

摘　　要：为了探究鱼源枯草芽孢杆菌J5在不同发酵条件下对棉粕中抗营养因子去除效果和棉粕营养组分影响,以鱼源枯草芽孢杆菌J5为发酵菌种,依据温度、湿度、接种量和发酵时间设计4因素3水平正交试验L9(3^(4))。结果表明,温度30℃,湿度60%,接种量5%,发酵4 d,游离棉酚脱毒率最高达94.8%,植酸含量显著下降,同时发酵后棉粕中粗蛋白含量显著上升。以游离棉酚、植酸、粗蛋白主效应分析结果得到的3组发酵条件发酵后,与未发酵组相比发酵棉粕中氨基酸含量和组成均优于其他组别。因此,鱼源枯草芽孢杆菌J5对棉粕最适发酵条件为温度30℃、湿度60%、接种量5%、发酵时间4 d。In order to investigate the effect of fish-derived Bacillus subtilis J5 on the elimination of anti-nutritional factors and the nutritional composition of cottonseed meal under different fermentation conditions,Bacillus subtilis J5 from the gut of common carp was used as a fermentation strain in the experiment.An orthogonal experiment L9(3^(4)),four-factors and three-levels was designed to obtain the optimal combination of temperature,humidity,inoculation amount,and fermentation duration for the best fermentation effect of cottonseed meal.The results showed that the highest detoxification rate of free gossypol in the cottonseed meal was achieved when the humidity was 60%,and the inoculation amount was 5%at 30℃for 4 d fermentation.Under this condition,94.8%free gossypol in the cottonseed meal was eliminated with a significant decrease of phytic acid content while the crude protein content of cottonseed meal significantly increased.The composition and amount of amino acids in the fermented cottonseed meal groups were superior to those in the unfermented group.In conclusion,the optimum fermentation conditions of Bacillus subtilis J5 for cottonseed meal were 60%humidity combined with 5%inoculation amount at 30℃for 4 d fermentation.

关 键 词：发酵棉粕 枯草芽孢杆菌J5 正交试验 棉酚 

分 类 号：S963.5[农业科学—水产养殖]