海州湾浮游细菌群落多样性  

Diversity of Planktonic Bacterial Communities in Haizhou Bay

作　　者：萧荣达 朱宇昂 狄美琪 李慧芳 徐军田 XIAO Rongda;ZHU Yuang;DI Meiqi;LI Huifang;XU Juntian(School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏连云港222005

出　　处：《江苏海洋大学学报（自然科学版）》2024年第3期9-19,共11页Journal of Jiangsu Ocean University:Natural Science Edition

基　　金：自然资源部滨海盐沼湿地生态与资源重点实验室开放基金项目(KLCSMERMNR2021107);连云港市博士后科研资助项目(LYG20220003)。

摘　　要：海洋细菌作为海洋中上层水体中丰度最高的微型生物类群,主导了海洋中的固碳、硫循环、硝化和氨氧化等生物地球化学循环过程。海州湾作为南黄海西端的开放海湾,资源丰富,是江苏省四大渔场之一。目前对海州湾浮游细菌群落的空间分布仍缺乏较为系统的研究。利用高通量测序技术,对海州湾北部、南部及靠近外海的8个站位进行浮游细菌群落结构多样性调查。研究结果表明,海州湾北部的生物多样性与丰富度最高,而外海站位最低。在海州湾海域共发现43个门、373个科,其中红杆菌科(Rhodobacteraceae)、莫拉氏菌科(Moraxellaceae)、黄杆菌科(Flavobacteriaceae)和放线菌科(Actinomarinaceae)是海州湾海域的主要优势科。基于Bray-Curtis距离的β多样性分析显示海州湾南部和北部的细菌群落结构差异显著,通过线性判别分析(linear discriminant analysis effect size,LEfSe)共找出172个差异分类单元。进一步分析发现,环境因子差异显著影响海州湾区域的细菌群落组成,其中温度、pH和NH+4-N直接或间接地影响细菌生长代谢进而影响海州湾细菌的群落结构组成。Marine bacteria,as the most abundant microbiological group in the middle and upper waters of the ocean,dominate the biogeochemical cycles of carbon sequestration,sulfur cycle,nitrification and ammonia oxidation in the ocean.As the most western open bay of the South Yellow Sea,Haizhou Bay is rich in resources and is one of the four major fishing grounds in Jiangsu Province.However,there is still a lack of systematic research on the distribution of bacterial community in Haizhou Bay.In this study,high-throughput sequencing technology was used to investigate the community structure and diversity of bacteria at 8 stations in the northern and southern of Haizhou Bay.The results showed that the biodiversity and richness of bacteria were highest in the northern Haizhou Bay,while lowest in the offshore stations.A total of 43 phyla and 373 families were found in all the samples,among which Rhodobacteraceae,Moraxellaceae,Flavobacteriaceae and Actinomarinaceae were the dominant families.β-diversity analysis based on Bray-Curtis distance showed significant differences in bacterial community structure between the southern and northern parts of Haizhou Bay,172 different taxa were identified by LEfSe analysis.Mantel test showed that the differences of environmental factors significantly affected the composition of bacterial community in Haizhou Bay,in which temperature,pH and NH+4-N significantly affected the composition of bacterial community through directly or indirectly affected the growth and metabolism of bacteria.

关 键 词：细菌多样性 群落结构 海州湾 

分 类 号：Q938.8[生物学—微生物学]