南方科技大学冷冻电镜中心建设与管理  

Construction and Management of Cryo-Electron Microscopy Center of Southern University of Science and Technology

作　　者：马晓旻 和思淼 吴静 高远瞩 许淑嫚 王培毅 MA Xiaomin;HE Simiao;WU Jing;GAO Yuanzhu;XU Shuman;WANG Peiyi(Cryo-EM Center,Southern University of Science and Technology,Shenzhen 518055,Guangdong,China;Department of Biology,Southern University of Science and Technology,Shenzhen 518055,Guangdong,China)

机构地区：[1]南方科技大学冷冻电镜中心,广东深圳518055 [2]南方科技大学生命科学学院,广东深圳518055

出　　处：《实验室研究与探索》2024年第9期213-218,240,共7页Research and Exploration In Laboratory

基　　金：国家自然科学基金青年基金项目(32301001);深圳市自然科学基金基础研究面上项目(JCYJ20210324122803009)。

摘　　要：近十年来,300 kV冷冻电镜已成为生物大分子结构解析、材料形貌及成分分析的有力工具。南方科技大学冷冻电镜中心是国内拥有该类设备最多的科研实验室,而且是国内首家集中化、自动化、可视化管理的冷冻电镜中心。它的建成填补了中国南方地区无高端冷冻电镜的历史空白。结合学校冷冻电镜中心大型仪器设备的状况、开放共享管理系统及运行模式,从管理框架和开放共享等方面探讨了该中心的管理及运行机制,为粤港澳大湾区结构生物学领域发展提供了重要支撑。In the past decade,300 kV cryo-electron microscopy(cryo-EM)were a powerful tool for the structural analysis of biological macromolecules,material morphology,and composition analysis.The Cryo-EM Center at Southern University of Science and Technology(SUSTech)is the largest scientific research laboratory in China with such equipment,and it is also the first centralized,automated,and visually managed cryo-EM center in China.Its completion fills the historical gap of the lack of high-end cryo-EM in southern China.This article summarizes the status of large-scale instruments and equipment,open sharing management system,and operation mode of the Cryo-EM Center at SUSTech,explores the management and operation mechanism of the center from the aspects of management framework and open sharing,and introduces its important support for the development of structural biology in the Guangdong-Hong Kong-Macao Greater Bay Area.

关 键 词：大型仪器设备平台 开放共享 冷冻电镜 分级管理 

分 类 号：TH742[机械工程—光学工程] TN16[机械工程—仪器科学与技术] G482[机械工程—精密仪器及机械] G647[电子电信—物理电子学]