槟榔碱对SD大鼠肠道微生物与神经递质的影响  

Effects of arecoline on gut microbiota composition and neurotransmitters contents in SD rats

作　　者：王傲雪 赵超[1] WANG Aoxue;ZHAO Chao(School of Pharmaceutical Sciences/Ministry of Education Key Laboratory of Tropical Biological Resources,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学药学院/热带生物资源教育部重点实验室,海口570228

出　　处：《热带生物学报》2024年第5期567-576,共10页Journal of Tropical Biology

摘　　要：为了解槟榔碱对SD大鼠肠道微生物与神经递质的影响,连续28 d用含500、100、0(CK)mg·kg^(-1)氢溴酸槟榔碱的0.9%NaCl溶液分别灌胃SD大鼠,然后检测分析SD大鼠结肠内容物中肠道微生物、粪便中短链脂肪酸的含量、脑皮质与血清中神经递质的含量等。结果发现,槟榔碱促使SD大鼠肠道内乳酸杆菌属、双歧杆菌属等的相对丰度提高,肠道内乙酸、丙酸等的含量增加,促进脑皮质中兴奋性神经递质DA和5‐HT等含量增加、降低了抑制性神经递质GABA的含量,表明槟榔碱可以通过微生物-肠-脑调节SD大鼠的生理、行为,使SD大鼠出现好动、食欲下降、体质量降低等现象。An attempt was made to understand the effects of arecoline on gut microbiota composition and neurotransmitters contents in SD rats based on the microbiota-gut-brain axis.Three groups of SD rats were administered with arecoline hydrobromide(Ah)solution dissolved in 0.9%NaCl aqueous solution by gavage for 28 days,and the ratios of weight of Ah/rat were 500,100 and 0(control group)mg·kg^(-1)after administration.Then the composition of intestinal microbes in colon,the contents of short-chain fatty acids in feces and the contents of neurotransmitters in the cerebral cortex and serum were determined and analyzed.The results showed that the Ah,when administered to SD rats,increased relative abundance of Lactobacillus and Bifidobacterium,and the contents of acetic acid and propionic acid in the gut,and the contents of excitatory neurotransmitters like DA and 5-HT in the cerebral cortex,and reduced the content of GABA which is inhibitory neurotransmitters.These results indicate that arecolineAh regulates the physiological metabolism and behavior of SD rats through microbiota-gut-brain axis,leading to symptoms such as hyperactivity,loss of appetite,and weight loss in SD rats.

关 键 词：槟榔碱 肠道微生物 神经递质 微生物-肠-脑轴 

分 类 号：R285.5[医药卫生—中药学]