火龙果根际促生微生物筛选及其效应  

Screening of microorganisms promoting rhizosphere growth in dragon fruit

作　　者：龚文坤 伍巧慧 王钢 杨劲明 吕荣婷 王蓓蓓 GONG Wenkun;WU Qiaohui;WANG Gang;YANG Jinmimg;LÜ Rongting;WANG Beibei(School of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带农林学院,海口570228

出　　处：《热带生物学报》2024年第5期632-638,共7页Journal of Tropical Biology

基　　金：海南省重点研发计划项目(ZDYF2021XDNY279);国家自然科学基金项目(42367015);海南省自然科学基金高层次人才项目(320RC483,422RC778)。

摘　　要：利用固氮、解磷、解钾及产IAA(吲哚乙酸)培养基从火龙果根际土壤中筛选出具有相应能力的一株木霉和一株芽孢杆菌,利用盆栽实验测定其发酵液对火龙果的促生效应。盆栽实验结果表明,从火龙果根际土壤中筛选出具有溶磷、解钾、固氮、产IAA能力的菌株F6、B3,其中F6(加纳木霉)固氮、产IAA能力最强,B3(贝莱斯芽孢杆菌)溶磷、解钾能力最强。盆栽试验结果表明,接种菌剂显著提高火龙果农艺性状,其中复合菌剂(B3+F6)效果最明显,鲜质量增加量提高33.09%,干质量增加量提高23.11%。接种菌剂能改善土壤肥力,显著提高土壤有效磷、速效钾、有机质含量,接种单个菌株B3、F6处理相较于CK处理有效磷含量分别提高了14.49%、9.9%;速效钾含量提高了10.4%、16.47%;碱解氮含量提高了25.85%、14.35%;接种混合菌株B3+F6处理相较于CK处理有效磷、速效钾、有机质含量分别提高了37.02%、12.64%、16.77%。土壤理化性质与火龙果促生指标相关性分析结果表明,接种复合菌剂下,土壤速效钾含量与火龙果地上部鲜质量、地上部干质量、火龙果氮磷钾积累存在显著正相关。由以上可以得出,接种B3和F6复合菌剂对火龙果地上部分鲜质量、干质量及火龙果根系具有明显提升效果,对火龙果氮磷钾营养元素的累积有增效作用,同时具有提高土壤肥力的功效。A strain of Trichoderma and a strain of Bacillus were screened from the rhizosphere soil of dragon fruit(Hylocereus undulates Britt.&Rose)using nitrogen fixation,phosphorus solubilization,potassium solubilization,and IAA production media.The growth promoting effect of their fermentation broth on dragon fruit was determined through pot experiments.The results showed that strains F6(Trichoderma ghanense)and B3(Bacillus velezensis)were screened from the rhizosphere soil of dragon fruit,which had the ability to dissolve phosphorus and potassium,fix nitrogen,and produce IAA.Among them,F6 had the highest ability to fix nitrogen and produce IAA,while B3 had the highest ability to dissolve phosphorus and potassium.Inoculation with microbial agents significantly improved the agronomic traits of dragon fruit.The combined microbial agents(B3+F6)had the most significant effect,increasing the fresh weight by 33.09%and the dry weight by 23.11%.Inoculation with microbial agents can improve soil fertility,significantly increase the contents of available phosphorus,available potassium,and organic matter in the soil.Compared with CK treatment,inoculation with a single strain B3 or F6 increased the available phosphorus content by 14.49%and 9.9%,the available potassium content by 10.4%and 16.47%,and the alkaline nitrogen content by 25.85%and 14.35%,respectively,whereas the inoculation of the mixed strains B3+F6 increased the contents of available phosphorus,available potassium,and organic matter by 37.02%,12.64%,and 16.77%,respectively.Correlation analysis between soil physicochemical properties and growth promoting indicators of dragon fruit shows that under the inoculation with the combined microbial agents the content of soil available potassium is significantly positively correlated with the fresh weight and dry weight of dragon fruit above ground,as well as the accumulation of nitrogen,phosphorus,and potassium in dragon fruit.It can hence be concluded that inoculation with the combined microbial agents(B3+F6)has a significant i

关 键 词：火龙果 促生微生物 解磷 固氮 解钾 

分 类 号：S154.3[农业科学—土壤学]