健脾清热活血方对胃癌细胞的干预作用及机制研究  

Intervention effect of Jianpi Qingre Huoxue Formula on gastric cancer cells

作　　者：文艺[1] 江晓涛 杨泽虹 黄远程 聂课朝 谢嘉丽 李培武[1] 刘凤斌[1] WEN Yi;JIANG Xiaotao;YANG Zehong;HUANG Yuancheng;NIE Kechao;XIE Jiai;LI Peiwu;LIU Fengbin(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510400,China;The First Clinical School,Guangzhou University of Chinese Medicine,Guangzhou 510400;The Second Xiangya Hospital,Central South University,Changsha)

机构地区：[1]广州中医药大学第一附属医院,广州510400 [2]广州中医药大学第一临床医学院,广州510400 [3]中南大学湘雅二医院,长沙410011

出　　处：《北京中医药》2024年第9期973-980,共8页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(82104602);国家自然科学基金面上项目(82474404,82474408);广州市科技计划项目市校(院)企联合项目(2024A03J0408);广州市科技计划项目市校(院)企联合项目(2024A03J0408);广州市科技计划项目(2023A03J00632);刘凤斌广东省名中医传承工作室建设项目(粤中医办函〔2020〕1号);徐志伟广东省名中医工作室建设项目(粤中医办函〔2024〕1号);第七批全国老中医药专家学术经验继承工作项目(国中医药人教函〔2022〕76号)。

摘　　要：目的探索健脾清热活血方(JQH)对胃癌细胞的干预作用。方法通过CCK-8、凋亡流式检测、划痕实验、裸鼠皮下成瘤试验,研究JQH对胃癌细胞的干预作用,并利用网络药理学获取其可能作用机制。结果经CCK-8检测,JQH干预组在24、48、72 h的增殖率均低于空白组;经流式细胞仪检测,JQH干预组凋亡细胞百分比高于空白组;经划痕实验,JQH干预组胃癌细胞24、48 h的愈合百分比(迁移能力)均低于空白组;JQH干预组裸鼠皮下瘤体的体积及重量均低于空白组,中、高剂量组抑瘤效果与5-Fu阳性药物对照组相当。网络药理学研究显示JQH可通过多靶点干预胃癌细胞,其作用机制可能涉及磷酸化、蛋白磷酸化、细胞增殖及凋亡的调控等功能,可通过调控PI3K/AKT通路抑癌。结论JQH可通过多途径、多通路影响胃癌细胞的增殖、迁移和凋亡。Objective To explore the intervention effect of Jianpi Qingre Huoxue Formula(JQH)on gastric cancer cells.Methods The intervention effect of JQH on gastric cancer cells was investigated using CCK-8 assays,apoptosis flow cytometry,scratch assays,and nude mouse subcutaneous tumor formation tests.Network pharmacology was employed to identify its potential mechanisms of action.Results After CCK-8 detection,the proliferation rate of JQH intervention group was lower than blank group at 24,48 and 72 h.Flow cytometry showed that the percentage of apoptotic cells in JQH intervention group was higher than blank group.By scratch test,the healing percentage(migration ability)of gastric cancer cells in JQH intervention group at 24 and 48 h was lower than blank group.The volume and weight of subcutaneous tumor of nude mice in the JQH intervention group were lower than those in the blank group,the antitumor effect of medium and high dose groups was similar to that of 5-Fu group.Network pharmacology indicated that JQH could intervene in gastric cancer cells through multiple targets.Its mechanisms of action may involve regulation of phosphorylation,protein phosphorylation,cell proliferation,and apoptosis,and it may inhibit cancer by modulating the PI3K/AKT pathway.Conclusion JQH can affect the proliferation,migration and apoptosis of gastric cancer cells through multiple pathways and pathways.

关 键 词：胃癌 健脾清热活血方 抑癌作用 信号通路 

分 类 号：R285[医药卫生—中药学]