过敏原可诱导气道过敏性疾病患者血液中性粒细胞TLR7表达上调  

Allergens can induce up-regulation of TLR7 expression in blood neutrophils of patients with airway allergic diseases

作　　者：王建叔 王君灵 张慧云 何韶衡 WANG Jianshu;WANG Junling;ZHANG Huiyun;HE Shaoheng(Research Center for Allergy and Clinical Immunology,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China)

机构地区：[1]锦州医科大学附属第一医院变态反应与临床免疫研究中心,锦州121001

出　　处：《中国免疫学杂志》2024年第9期1919-1924,共6页Chinese Journal of Immunology

基　　金：国家重点自然科学基金项目(81030054);辽宁省过敏性疾病过敏原致病机制研究重点实验室建设项目(2018-30)。

摘　　要：目的:检测过敏原激发前后过敏性鼻炎(AR)、过敏性哮喘(AA)及过敏性鼻炎合并哮喘(ARA)患者外周血中性粒细胞Toll样受体7(TLR7)的表达变化。方法:本研究募集19例健康人(HC)、44例AR、36例AA和19例ARA患者,取各组外周血,采用大籽蒿过敏原提取物(ASWE)、屋尘螨过敏原提取物(HDME)和梧桐花粉过敏原提取物(PPAE)进行激发试验,流式细胞术检测中性粒细胞TLR7表达情况。结果:在静息状态下,与HC相比,气道过敏患者中性粒细胞TLR7表达上调,其中AR、AA及ARA患者TLR7+中性粒细胞比例分别升高6倍、3.18倍、6.15倍,ARA患者中性粒细胞TLR7平均荧光强度(MFI)增强24%。过敏原激发试验结果显示,上述过敏原激发前后,HC组中性粒细胞TLR7的表达无明显变化,而气道过敏患者中性粒细胞TLR7表达上调。HDME、PPAE激发后AR患者外周血TLR7+中性粒细胞百分比分别升高8.40%、33.03%,TLR7的MFI分别增强29.62%、35.72%。ASWE、PPAE激发后,AA患者外周血TLR7+中性粒细胞百分比分别增加59.18%、1.06倍,TLR7的MFI分别增强68.93%、47.33%。而HDME激发仅引起AA患者中性粒细胞TLR7的MFI增强66.14%;HDME激发后ARA患者外周血中性粒细胞内TLR7的百分比和MFI分别增加21.05%、35.61%,而PPAE激发后仅伴有ARA患者TLR7+细胞百分比升高20.07%。结论:过敏性气道疾病患者经过敏原激发后血液中性粒细胞TLR7表达升高,提示过敏原可能通过诱导中性粒细胞中TLR7表达变化参与过敏性气道疾病的发生发展过程。Objective:To detect the expression of Toll-like receptor 7(TLR7)in blood neutrophils of patients with allergic rhinitis(AR),allergic asthma(AA)and allergic rhinitis combined with allergic asthma(ARA)before and after allergen challenge.Methods:A total of 44 AR,36 AA,19 ARA patients and 19 healthy control(HC)were recruited,the extracts of Artemisia siever⁃siana wild allergen(ASWE),house dust mite allergen(HDME)and Platanus pollen allergen(PPAE)were used to challenge peripheral blood of HC as well as the patients,the expression of TLR7 in neutrophils was detected by flow cytometry.Results:In a resting state,compared with HC,upregulated expression of TLR7 in neutrophils in patients with airway allergy:the percentage of TLR7+neutrophils in AR,AA,ARA patients increased 6 times,3.18 times and 6.15 times,respectively,and the mean fluorescence intensity(MFI)of TLR7 expression in neutrophil in ARA was enhanced by 24%.The results of allergen challenge tests showed that the expression of neutrophils TLR7 did not change in HC subjects after the allergen challenge,whereas the expression of TLR7 in neutrophils of patients with airway allergy was upregulated after the allergens challenge.After HDME and PPAE challenge,the percentage of TLR7+neutrophils in peripheral of AR patients increased by 8.40%and 33.03%,respectively,and the MFI of TLR7 enhanced by 29.62%and 35.72%,respectively.After ASWE and PPAE challenge.The percentage of TLR7+neutrophils in peripheral of AA patients increased by 59.18%and 1.06 times,respectively,and the MFI of TLR7 enhanced by 68.93%and 47.33%,respectively.However,after HDME stimulation only enhanced the MFI of neutrophil TLR7 in AA patients by 66.14%;after HDME challenge,the percentage of TLR7 and MFI in peripheral blood neutrophils of patients with ARA increased by 21.05%and 35.61%,respectively,while after PPAE challenge,the percentage of TLR7+cells in patients with ARA increased by 20.07%.Conclusion:The expression of TLR7 in blood neutrophils is increased in patients with allergic airway diseases af

关 键 词：过敏性鼻炎 过敏性哮喘 过敏性鼻炎合并哮喘 中性粒细胞 TLR7 

分 类 号：R765.21[医药卫生—耳鼻咽喉科] R256.12[医药卫生—临床医学] R392.8