CD19^(+)CD24^(hi)CD27^(+)调节性B细胞水平与强直性脊柱炎间的关系探讨  

Association of CD19^(+)CD24^(hi)CD27^(+)regulatory B cells with ankylosing spondylitis

作　　者：戴薇[1] 刘玉兰 曾艳梅 李世云[1] DAI Wei;LIU Yulan;ZENG Yanmei;LI Shiyun(Department of Laboratory Medicine,Ganzhou People's Hospital,Ganzhou 341000,China;Central Laboratory,Ganzhou People's Hospital,Ganzhou 341000,China)

机构地区：[1]赣州市人民医院检验科,赣州341000 [2]赣州市人民医院中心实验室,赣州341000

出　　处：《中国免疫学杂志》2024年第9期1940-1943,共4页Chinese Journal of Immunology

摘　　要：目的:探究CD19^(+)CD24^(hi)CD27^(+)调节性B细胞(Bregs)水平与强直性脊柱炎(AS)的关系。方法:将赣州市人民医院2019年1月至2021年12月间收治的80例AS患者纳为观察组,同期体检合格的健康志愿者60例纳为对照组。根据观察组患者疾病临床分期将其分为进展期及强直期,根据患者疾病活动度将其分为活动组、稳定组,检测并比较观察组及对照组、观察组不同分期患者外周血CD19^(+)CD24^(hi)CD27^(+)Breg占CD19^(+)细胞百分比。分析CD19^(+)CD24^(hi)CD27^(+)Bregs百分比与AS患者病程、晨僵时间、巴斯强直性脊柱炎疾病活动指数评分(BASDAI)、IL-10、血沉(ESR)、C反应蛋白(CRP)及骶髂关节X线片分级等临床特点之间的关系。结果:观察组AS患者外周血CD19^(+)CD24^(hi)CD27^(+)Bregs占CD19^(+)B细胞百分比高于健康对照组,强直期AS患者CD19^(+)CD24^(hi)CD27^(+)Breg占CD19^(+)细胞百分比高于进展期患者,且骨性强直期患者外周血CD19^(+)CD24^(hi)CD27^(+)Breg百分比高于纤维性强直期患者,活动组AS患者外周血CD19^(+)CD24^(hi)CD27^(+)Breg占比高于稳定组,以上差异均有统计学意义(P<0.05)。观察组血清IL-10水平低于对照组,ESR及CRP水平高于对照组,差异有统计学意义(P<0.05)。AS患者外周血CD19^(+)CD24^(hi)CD27^(+)Breg占CD19^(+)B细胞百分比与其BASDAI得分及血清IL-10水平呈正相关,与ESR及CRP水平呈负相关,与患者晨僵时间及髂关节X线分级无明显相关性。结论:AS患者外周血CD19^(+)CD24^(hi)CD27^(+)Breg占CD19^(+)B细胞百分比降低,且其水平与患者疾病分期、活动度及实验室指标IL-10、ESR及CRP间具有一定的相关性。Objective:To analyze the association of CD19^(+)CD24^(hi)CD27^(+)regulatory B cells(Bregs)with ankylosing spondylitis(AS).Methods:Eighty patients with AS in Ganzhou People's Hospital from January 2019 to December 2021 were enrolled as observation group,meantime,another 60 healthy individuals were set as control group.Observation group was further divided into subgroups according to clinical stages and disease activity,advanced stage,ankylosis stage,active stage and non-active stage.Percentage of CD19^(+)CD24^(hi)CD27^(+)Bregs in CD19^(+)cells in peripheral blood of observation group and control group,and observation group patients with different stages were detected and compared,thereafter,relationship between percentage of CD19^(+)CD24^(hi)CD27^(+)Bregs and clinical characteristics of AS patients were discussed,such as course of disease,duration of morning stiffness,the Bath Ankylosing Spondylitis Disease Activity Index(BASDAI),IL-10,erythrocyte sedimentation rate(ESR),C-reactive protein(CRP)and X-ray classification of sacroiliitis.Results:Percentage of CD19^(+)CD24^(hi)CD27^(+)Bregs in peripheral blood in observation group was higher than that in control group.Percentage of CD19^(+)CD24^(hi)CD27^(+)Bregs in CD19^(+)cells in AS patients at ankylosis stage was higher than that in patients at advanced stage,and percentage of CD19^(+)CD24^(hi)CD27^(+)Bregs in peripheral blood of patients with bony ankylosis stage was higher than that in patients with fibrous ankylosis stage.Percentage of CD19^(+)CD24^(hi)CD27^(+)Breg in CD19^(+)B cells in active AS patients was significantly higher than that of patients with stable AS(P<0.05).Serum IL-10 level in observation group was lower than that in control group,while ESR and CRP levels were higher than those in control group,with statistical significance(P<0.05).Percentage of CD19^(+)CD24^(hi)CD27^(+)Breg in CD19^(+)B cells in peripheral blood of AS patients was positively correlated with BASDAI score and serum IL-10 level,while negatively correlated with ESR and CRP levels,a

关 键 词：CD19^(+)CD24^(hi)CD27^(+)调节性B细胞 强直性脊柱炎 临床分期 疾病活动度 

分 类 号：R446.9[医药卫生—诊断学]