病毒样颗粒常用表达系统及应用研究进展  

Research progress on commonly used expression systems and applications of virus-like particles

作　　者：舒星富 陈遥 苏津贤 马小梅 柏家林[1] 马忠仁[1] 张海霞[1] SHU Xingfu;CHEN Yao;SU Jinxian;MA Xiaomei;BAI Jialin;MA Zhongren;ZHANG Haixia(BioMedical Research Center,Key Laboratory of Bioengineering and Biotechnology of the State Ethnic Affairs Commission,Northwest Minzu University,Lanzhou 730030,China;Life Science and Engineering College,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室,兰州730030 [2]西北民族大学生命科学与工程学院,兰州730030

出　　处：《中国免疫学杂志》2024年第10期2194-2201,共8页Chinese Journal of Immunology

基　　金：甘肃省自然科学基金项目(23JRRA717);2023年度甘肃省教育厅高等学校创新基金项目(2023B-059);西北民族大学中央高校基本科研业务费专项资金资助项目(31920230165)。

摘　　要：病毒样颗粒(VLPs)是由一种或多种病毒结构蛋白自组装而成的纳米颗粒,这些结构蛋白可以排列成几层,也可以包含脂质外膜。由于缺乏遗传物质,因此VLPs无法感染宿主细胞,但其具有高度的免疫原性,能够诱导不同于常规灭活疫苗的免疫应答。VLPs可利用包括细菌、酵母、植物、昆虫和哺乳动物细胞等多种系统进行生产。与传统疫苗相比,VLPs具有不可比拟的优势,因此在生物医学领域越来越受欢迎。迄今为止,已开发出一系列基于VLPs的候选疫苗,用于免疫和预防各类传染病。同时,最近VLPs因在靶向药物传递和基因治疗的成功应用而受到关注。本文主要综述了VLPs的常用表达系统及应用研究进展。Virus-like particles(VLPs)are nanoparticles that are self-assembled from one or more structural proteins,which can be arranged in several layers or contain a lipid outer membrane.Due to the lack of genetic material,VLPs cannot infect host cells,but are highly immunogenic and can induce immune responses different from conventional inactivated vaccines.VLPs can be produced using a variety of systems including bacterial,yeast,plant,insect and mammalian cells.Compared with traditional vaccines,VLPs have incomparable advantages,so they are becoming more and more popular in the biomedical field.To date,a series of vaccine candi⁃dates based on VLPs have been developed for immunization and prevention of various infectious diseases.At the same time,the recent successful application of VLPs in targeted drug delivery and gene therapy has attracted attention.This paper mainly reviews the com⁃monly used expression systems of VLPs and the research progress of their applications.

关 键 词：病毒样颗粒 表达系统 疫苗 药物传递 

分 类 号：Q818[生物学—生物工程]