新型均相免疫分析技术——荧光淬体免疫探针分析  

A novel homogeneous immunoassay technique——Quenchbody assay

作　　者：赵广伟 陈浩 董金华 ZHAO Guangwei;CHEN Hao;DONG Jinhua(School of Life Science and Technology,Shandong Second Medi-cal University,Weifang 261053,China;School of Basic Medical Science,Shandong University,Jinan 250012,China;Biological Science Research Center,Southwest University,Chongqing 400715,China;School of Reha-bilitation Science and Engineering,University of Health and Rehabilitation Science,Qingdao 266113,China)

机构地区：[1]山东第二医科大学生命科学与技术学院,潍坊261053 [2]山东大学基础医学院,济南250012 [3]西南大学生物学研究中心,重庆400715 [4]康复大学康复科学与工程学院,青岛266113

出　　处：《中国免疫学杂志》2024年第10期2223-2228,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金(21775064)。

摘　　要：生化分析在疾病诊断、环境污染源鉴定、食品安全等多个领域具有重要意义。基于抗原抗体反应的免疫检测技术具有高特异性与多样性等特点,其操作比基于大型设备的色谱法简单,检测对象选择性更好,广泛应用于样品检测。荧光淬体免疫探针(Q-body)是一种通过荧光染料标记抗体片段构建成的新型荧光免疫检测探针,抗体内部色氨酸导致荧光基团发生电子转移而淬灭,当与抗原特异性结合时,其荧光强度恢复并呈浓度依赖性。因此,可通过检测Q-body的荧光强度变化检测抗原浓度。Q-body免疫检测技术因操作简便及灵敏度高等特点,被广泛应用于环境污染物质及疾病标志物的快速检测,对环境检测及疾病诊断具有重要意义。本文从Q-body的原理、构建方法、检测技术的开发及国内外研究进展进行综合论述,并对该技术的未来应用做出展望。Biochemical analysis is of great significance in many fields,such as disease diagnosis,environmental pollution source identification,food safety,etc.Immunoassay based on the reaction of antigen and antibody has the characteristics of high speci⁃ficity and diversity.Compared with other analytical methods such as liquid chromatography,which requires a large and expensive in⁃strument,immunodetection has the advantages of simplicity and good selectivity and is thus widely used in sample testing.Quench⁃body(Q-body)is a novel fluorescent immunosensor based on antigen-antibody reaction.It is constructed by labeling antibody fragment with fluorescent dye.Tryptophan in antibody leads to electron transfer and quenching of fluorescent,when it specifically binds with an⁃tigen,its fluorescence intensity recovers and presents concentration dependence.Therefore,the antigen concentration can be detected by detecting the change of fluorescence intensity of the Q-body.Q-body immunoassay is widely used in the rapid detection of environ⁃mental pollutants and disease markers due to its simple operation and high sensitivity,which is of great significance for environmental detection and disease diagnosis.In this paper,the principle,construction method,detection technology development and domestic and foreign research progress of Q-body are comprehensively discussed,and the future application of this technology is prospected.

关 键 词：抗体 免疫检测 荧光探针 淬灭 疾病标志物 

分 类 号：R446.61[医药卫生—诊断学]