红胸黑翅萤线粒体全基因组测定及熠萤亚科系统发育树分析  

The Complete Mitochondrial Genome of Luciola kagiana Matsumura,1928(Coleoptera:Lampyridae)and Phylogenetic Analysis of Luciolinae

作　　者：陈泉 刘勇[2] 刘全 付新华[3] CHEN Quan;LIU Yong;LIU Quan;FU Xinhua(Kaisa Charity Foundation,Shenzhen,518000;Shandong Huayang Technology Co.,Ltd.,Tai′an,271400;College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,430070)

机构地区：[1]深圳市佳兆业公益基金会,深圳518000 [2]山东华阳农药化工集团有限公司,泰安271400 [3]华中农业大学植物科学技术学院,武汉430070

出　　处：《基因组学与应用生物学》2024年第7期1260-1274,共15页Genomics and Applied Biology

基　　金：国家自然科学基金项目(32070485)资助。

摘　　要：为明确红胸黑翅萤(Luciola kagiana Matsumura,1928)(Coleoptera:Lampyridae)的线粒体基因组结构及熠萤亚科的分子系统发育关系,本研究对红胸黑翅萤的线粒体基因进行了测序、组装、注释和特征分析。结果表明,红胸黑翅萤的线粒体基因组全长为16317 bp,包含了13个蛋白编码基因(proteincoding genes,PCGs)、22个tRNA基因、2个rRNA基因和一个非编码控制区。对21种萤火虫(其中19种为熠萤亚科,2种为萤亚科)的线粒体基因组的组成和结构进行分析,分析结果表明,萤科(Lampyridae)昆虫线粒体基因组组成和结构的基本特征一致,但在线粒体全长、控制区长度、A+T含量偏向性以及基因间隔特征上在亚科间存在一定的差异性。利用最大似然法构建基于这21种萤火虫的13个PCGs的系统发育树,结果显示,红胸黑翅萤与熠萤属(Luciola)萤火虫聚类在一个分支上;熠萤亚科与萤亚科萤火虫各自聚成一个分支;熠萤亚科间同属的萤火虫种类都分别单独形成一个分支。本研究表明分子分类技术能极大地促进萤火虫分类学系统的完善,提升萤火虫分类的便利性和准确性。In order to understand the mitochondrial genome structure of Luciola kagiana Matsumura,1928(Coleoptera:Lampyridae)and phylogenetic analysis of Luciolinae,the mitochondrial genome of L.kagiana was sequenced,and the genome sequences were then assembled,annotated and characterized.The results showed that the complete mitochondrial genome of L.kagiana was 16317 bp in length,including 13 proteincoding genes(PCGs),22 tRNA genes,2 rRNA genes,and one control region.At the same time,the composition and structure of the mitochondrial genomes of 21 fireflies(including 19 species belonging to the subfamily Luciolinae and 2 species belonging to the subfamily Lampyrinae)were analyzed.It was found that the basic features of mitochondrial genome composition and structure remained consistent in Lampyridae,but there was some variability under subfamilies in whole genome,length of control region,A+T skew and intergenic sequences.And the phylogenetic relationship was constructed by using maximum likelihood method based on the 13 PCGs of 21 species of fireflies.The results showed that L.kagiana gathered together with other two firefly species of Luciola on a branch,and the fireflies of Luciolinae and Lampyrinae each gathered together on a branch,and the fireflies of the same genus under the Luciolinae were clustered on a branch,respectively.This study shows that the development of molecular classification technology can assist and promote the systematization and accuracy of traditional morphological classification.

关 键 词：红胸黑翅萤 熠萤属 熠萤亚科 形态学分类 系统发育 线粒体基因组 

分 类 号：Q963[生物学—昆虫学]