Genome-Wide Identification of ABCC Gene Subfamily Members and Functional Analysis of CsABCC11 in Camellia sinensis  

作　　者：Mingyuan Luo Shiyu Tian Xinzhuan Yao Yue Wan Zhouzhuoer Chen Zifan Yang Huagen Hao Fei Liu Hu Tang Litang Lu 

机构地区：[1]The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Science,Guizhou University,Guiyang,550025,China [2]College of Tea Science,Institute of Plant Health&Medicine,Guizhou University,Guiyang,550025,China [3]Wengfu Group,Guiyang,550025,China [4]Huaneng Clean Energy Research Institute,Guiyang,550000,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第8期2019-2036,共18页国际实验植物学杂志（英文）

基　　金：supported by the Guizhou University Talent Introduction Program([2021]05);Guizhou University Cultivation Program([2020]48);Institute of Technology of YF([2022]017);Guizhou Province High-Level Innovative Talents“Hundred”Level Talent Project(Qiankehe Platform Talent)GCC[2023]014;Supported by the earmarked fund for GZMARS-Tea and Research on the Planting Technology of China HUANENG Photovoltaic Tea Garden(Project No.HNKJ2022-H135).

摘　　要：The ATP-binding cassette(ABC)transporter is a gene superfamily in plants.ATP-binding cassette subfamily C(ABCC)protein is a multidrug resistance-associated(MRP)transporter.They play various roles in plant growth,development,and secondary metabolite transport.However,there are few studies on ABCC transporters in tea plants.In this study,genome-wide association study(GWAS)analysis of epigallocatechin gallate(EGCG)content in 108 strains of Kingbird revealed that CsABCCs may be involved in EGCG transport.We identified 25 CsABCC genes at the genomic level of the tea plant,their phylogenetic tree,gene structure,targeted miRNA and other bioinformatics were analyzed.The expression patterns of CsABCCs in eight different tissues and abiotic stress indicate that they have potential roles in regulating the growth,development,and defense of tea plants.The correlation analysis revealed that the expression of the CsABCC11 gene was closely related to the EGCG content in tea buds of 108 strains of the Kingbird,and the subcellular localization experiments in tobacco showed that CsABCC11 protein was localized on the plasma membrane.The virus-induced gene silencing(VIGS)strategy in tea plants further verified that CsABCC11 was involved in EGCG accumulation.Our study laid a foundation for studying the biological function of CsABCC and provided a new candidate molecular marker gene for further EGCG-related variety breeding,which will be of great interest to breeders.

关 键 词：Camellia sinensis ABCC gene subfamily EGCG VIGS guizhou local variety Kingbird 

分 类 号：Q943.2[生物学—植物学]