基于转录组学挖掘湘巨1号耐盐相关候选基因  

Exploring candidate genes related to salt tolerance of Xiangju 1 based on transcriptomics

作　　者：夏敏 徐楠 朱倩锋 陈彦超 杜丽桦 汪启明[1] 夏新界[2] 饶力群[1] XIA Min;XU Nan;ZHU Qian-feng;CHEN Yan-chao;DU Li-hua;WANG Qi-ming;XIA Xin-jie;RAO Li-qun(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410000,China)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]中国科学院亚热带农业生态研究所,长沙410000

出　　处：《西南农业学报》2024年第8期1645-1654,共10页Southwest China Journal of Agricultural Sciences

基　　金：湖南省发改委科技专项([2016]245号)。

摘　　要：【目的】研究巨型稻幼苗期响应盐胁迫差异基因的表达特异性,解析巨型稻耐盐机理及分子机制,为水稻耐盐性遗传改良提供理论依据。【方法】以湘巨1号为材料,设置对照和1个盐浓度水平(0.5%NaCl),处理72 h,随后应用转录组测序技术分析盐胁迫下响应基因的动态变化与主要富集途径。【结果】盐处理后湘巨1号幼苗的苗高和鲜重均受到低于30%的胁迫损害。每个样本共获得的干净片段为58.93 G Clean bases,平均GC含量为53.51%,平均Q20和Q30分别为97.84%和93.89%。基因差异表达分析筛选出2327个差异基因,其中1363个上调表达基因,964个下调表达基因。对差异基因进行功能注释分析发现,GO富集分析结果显示差异基因显著富集在166个GO条目中,其中最显著富集的条目分别是生物过程中的氧化还原过程、核代谢过程、转录调控等;细胞组分中的膜组分;分子功能中的氧化还原酶活性、单氧酶活性、转录因子活性等。KEGG分析结果表明,差异基因显著富集在次生代谢物合成、植物激素信号转导、苯丙烷生物合成、谷胱甘肽代谢等途径。【结论】湘巨1号具有较强耐盐性,GO分析表明湘巨1号通过调控抗氧化酶活性和转录因子表达来响应盐胁迫。KEGG分析表明植物激素信号转导、苯丙烷生物合成、谷胱甘肽代谢等途径在响应盐胁迫中起主导作用。本研究利用RNA-Seq测序技术筛选水稻响应盐胁迫的候选基因,为深入研究巨型稻耐盐调控机制提供基础,也为后续新品种研发培育提供重要理论依据。【Objective】The present paper aimed to analyze the salt tolerance mechanism and molecular mechanism of giant rice by analyzing the expression specificity of differential genes in response to salt stress at seedling stage,and to provide a theoretical basis for genetic improve⁃ment of salt tolerance of giant rice.【Method】Xiangju 1 was used as the material,and the control and one salt concentration level(0.5%NaCl)were set up and treated for 72 hours.The dynamic changes and main enrichment pathways of response genes under salt stress were an⁃alyzed by transcriptome sequencing.【Result】After salt stress,seedling height and fresh weight of Xiangju 1 were damaged by less than 30%stress.The total clean reads per each sample were 58.93 G Clean bases,the average GC content per each sample was 53.51%,the average Q20 and Q30 per each sample were 97.84%and 93.89%respectively.Gene differential expression analysis identified 2327 differentially ex⁃pressed genes,including 1363 up⁃regulated genes and 964 down⁃regulated genes.The differentially expressed genes were analyzed by func⁃tional annotation,GO enrichment analysis showed that they were significantly enriched in 166 GO terms,among which the most significantly enriched terms were biological process of oxidation reduction process,nuclear metabolism process,transcriptional regulation;The cellular com⁃ponent of the cell component;The molecular function of the oxidoreductase activity,monooxygenase activity,transcription factor activity,etc.KEGG analysis showed that differential genes were significantly enriched in secondary metabolite synthesis,plant hormone signal transduc⁃tion,phenylpropanoid biosynthesis,and glutathione metabolism.【Conclusion】Xiangju 1 has strong salt tolerance,GO analysis shows that Xiangju 1 responds to salt stress by regulating antioxidant enzyme activity and transcription factor expression.KEGG analysis shows that plant hormone signal transduction,phenylpropanoid biosynthesis,glutathione metabolism and other pathways play a l

关 键 词：巨型稻 苗期 耐盐性 转录组 

分 类 号：S511[农业科学—作物学]