绵羊LEP、LEPR基因多态性与体况评分的相关性分析  

Correlation analysis between LEP,LEPR gene polymorphism and body condition score in sheep

作　　者：焦文静 梁龙 海拉提·卡斯 刘宜勇 王钰琪 岳晨冰 巴德玛 贺三刚 JIAO Wen-jing;LIANG Long;HAILATI-Kasimu;LIU Yi-yong;WANG Yu-qi;YUE Chen-bing;BADEMA;HE San-gang(Institute of Biotechnology,Xinjiang Academy of Animal Husbandry Sciences,Key Laboratory of Genetic Breeding and Reproduction of Herbivorous Livestock,Ministry of Agriculture and Rural Affairs,Xinjiang Key Laboratory of Animal Biotechnology,Urumqi,830011,China;Yili Kazak Autonomous Prefecture Animal Husbandry Station,Yining,Xinjiang 835000,China;College of Animal Science,Xinjiang Ag-ricultural University,Urumqi 830052,China;Zhaosu Horse Farm in Ili Kazak Autonomous Prefecture,Zhaosu,Xinjiang 835500,China)

机构地区：[1]新疆畜牧科学院生物技术研究所,农业农村部草食家畜遗传育种与繁殖重点实验室,新疆动物生物技术重点实验室,乌鲁木齐830011 [2]伊犁哈萨克自治州畜牧总站,新疆伊宁835000 [3]新疆农业大学动物科学学院,乌鲁木齐830052 [4]伊犁哈萨克自治州昭苏马场,新疆昭苏835500

出　　处：《西南农业学报》2024年第8期1862-1870,共9页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2021YFD1300903);新疆维吾尔自治区重大科技专项(2022A02001-2);新疆维吾尔自治区“三农”骨干人才培养项目(2023SNGGCY010)。

摘　　要：【目的】研究特克赛尔×哈萨克杂交(特哈)母羊瘦素(Leptin,LEP)、瘦素受体(Leptin receptor,LEPR)基因多态性(Single nucleotide polymorphisms, SNPs)及其与体况评分(Body condition score, BCS)之间的相关性。【方法】采用PCR测序技术筛选LEP和LEPR第18外显子中的错义突变,用KASP分型方法进行SNPs分型,并将分型结果与其BCS关联分析。【结果】①在特哈母羊LEP基因上共发现1个SNPs位点,即rs1093355763,在特哈母羊LEPR基因上共发现3个SNPs位点,即rs428867159、rs412929474和g.2770G>A,均属于外显子错义突变,rs428867159和rs412929474属于中度多态(0.25A属于低度多态(PIC≤0.25),均处于Hard-Weinberg平衡状态(P≤0.05)。②rs1093355763位点的基因型效应中,特哈母羊哺乳中期时CA基因型的体重显著高于CC基因型(P<0.05,下同)。rs428867159位点的基因型效应中,特哈母羊产羔期时TT基因型的BCS显著高于CC基因型和CT基因型。g.2770G>A位点的基因型效应中,特哈母羊妊娠120 d时GA基因型的BCS显著高于GG基因型,特哈母羊断奶期时GG基因型的BCS显著高于GA基因型。特哈母羊妊娠120 d时GA基因型的体重显著高于GG基因型。【结论】LEP和LEPR基因中rs1093355763、rs428867159和g.2770G>A位点可以作为特哈母羊BCS和体重的分子标记应用于绵羊育种。【Objective】The study aimed to analyze the single nucleotide polymorphisms(SNPs)of LEP and LEPR genes and their correlation with body condition score(BCS)in Texel×Kazak hybrid(Teha)ewes.【Method】The missense mutations in exon 18 of LEP and LEPR were screened by PCR sequencing technology,and the SNPs were genotyped by KASP typing method,and the association between the genoty⁃ping results and their BCS was analyzed.【Result】(i)The results showed that one SNP site,rs1093355763,was found in the LEP gene,and three SNPs sites,rs428867159,rs412929474 and g.2770 G>A,were found in the LEPR gene.All of them belong to exon missense muta⁃tions,rs428867159 and rs412929474 belonged to moderate polymorphism(0.25<PIC<0.5),and rs1093355763 and g.2770 G>A be⁃longed to low polymorphism (PIC≤0. 25). All of them were in Hard⁃Weinberg equilibrium (P ≤0. 05). (ii) In the genotype effect ofrs1093355763,the body weight of CA genotype was significantly higher than that of CC genotype at the middle stage of lactation (P <0. 05,the samw as below). Among the genotype effects of rs428867159,the BCS of TT genotype was significantly higher than that of CC genotypeand CT genotype at lambing stage. In the genotype effect of g. 2770G > A locus,the BCS of GA genotype was significantly higher than thatof GG genotype at 120 days of pregnancy,and the BCS of GG genotype was significantly higher than that of GA genotype at weaning stage.The body weight of GA genotype was significantly higher than that of GG genotype at 120 days of pregnancy. 【Conclusion】Thers1093355763,rs428867159 and g. 2770 G > A loci in LEP and LEPR genes can be used as molecular markers for BCS and body weight ofTeha ewes in sheep breeding.

关 键 词：BCS LEP基因 LEPR基因 核苷酸多态性 相关性分析 

分 类 号：S858.26[农业科学—临床兽医学]