赤水乌骨鸡线粒体Cytb基因全序列遗传和起源分析  

Genetic and original analysis of full sequence of mitochondrial DNA Cytb gene in Chishui black bone chicken

作　　者：吴芸[1] 李盼盼 闫吉美[1] 黄锡阳 WU Yun;LI Pan-pan;YAN Ji-mei;HUANG Xi-yang(School of Biology and Agriculture Technology,Zunyi Normal University,Zunyi,Guizhou 563006,China;Animal Husbandry and Fishery Station of Chishui Agriculture and Rural Bureau,Chishui,Guizhou 564700,China)

机构地区：[1]遵义师范学院生物与农业科技学院,贵州遵义563006 [2]赤水市农业农村局畜牧渔业站,贵州赤水564700

出　　处：《西南农业学报》2024年第8期1871-1875,共5页Southwest China Journal of Agricultural Sciences

基　　金：遵义师范学院2021年乡村振兴专项项目(黔教合KY字[2012]017-17)。

摘　　要：【目的】探明赤水乌骨鸡品种群体的遗传多样性和起源进化特点,为赤水乌骨鸡的种质资源评估,遗传资源保护和利用提供理论依据。【方法】采用PCR扩增及测序技术对60只赤水乌骨鸡的Cytb基因进行全长序列研究,采用生物信息学方法进行遗传多样性及起源进化分析。【结果】赤水乌骨鸡Cytb基因序列全长为1143 bp,碱基T、C、A和G的平均占比分别是24.1%、36.4%、27.5%和12.0%。Cytb基因序列分析发现共有13个突变位点,均为颠换,单倍型多样性(Hd)为0.796,核苷酸多样性(Pi)是0.001 91,平均核苷酸差异(K)为2.180。单倍型网络图显示在赤水乌骨鸡Cytb基因序列的9种单倍型中Hap3和Hap2为优势单倍型。赤水乌骨鸡Cytb基因9种单倍型与红色原鸡5个亚种Cytb基因序列构建的系统发育树表明Hap1与GGS12聚为一类,Hap2与GGM1聚为一类,Hap6、Hap7和Hap3聚在一起,与GGM3形成一个小分支,Hap8和Hap9与GGS3、GGS4和GGM2聚在一起形成一个分支,Hap4和Hap5则是自成一支。【结论】赤水乌骨鸡群体遗传多样性较高,可能起源于原鸡滇南亚种(Gallus gallus spadiceus)和原鸡印度亚种(Gallus gallus murghi)。【Objective】The genetic diversity and evolutionary characteristics of Chishui black bone chicken populations were studied,which provided theoretical basis for evaluation of germplasm resource of Chishui black bone chicken,preservation and utilization of genetic re⁃sources.【Method】The full sequence of Cytb gene of 60 Chishui black bone chickens were tested by PCR amplification and sequencing,and then the genetic diversity and evolution were analyzed by bioinformatics.【Result】The full⁃length of Cytb gene in Chishui black bone chicken were 1143 bp,with the proportions of 24.1%base T,36.4%C,27.5%A and 12%G respectively.A total of 13 variable sites were found and all were transversions.The haplotype diversity(Hd),nucleotide diversity(Pi)and average nucleotide differences(K)were 0.796,0.00191 and 2.180 respectively.The haplotype network showed that Hap3 and Hap2 were the dominant haplotypes in the whole 9 haplotypes of Cytb gene sequence in Chishui black bone chicken.The phylogenetic tree constructed by the 9 haplotypes of Cytb gene sequence in Chishui black bone chicken and Cytb gene sequence in 5 red jungle fowl subspecies suggested that Hap1 was clustered with GGS12,and Hap2 was clustered with GGM1.Hap6,Hap7 and Hap3 were clustered with GGM3 into one branch;Hap8 and Hap9 were clustered with GGS3;GGS4 was clustered with GGM2;Hap4 and Hap5 were clustered into another branch.【Conclusion】The genetic diversity of Chishui black bone chicken is high,which probably originates from Gallus gallus spadiceus and Gallus gallus murghi.

关 键 词：赤水乌骨鸡 MTDNA CYTB基因 遗传多样性 

分 类 号：S831.2[农业科学—畜牧学]