不同负压封闭引流压力与大鼠创面中厚皮片移植疗效的关系研究  

Study on the Relationship between Sealing Drainage Pressure with Differ⁃ent Negative Pressure and the Effect of Medium and Thick Skin Graft in Rat Wound

作　　者：黄志明 李自军 骆丽 赵俊延 赵文波 HUANG Zhiming;LI Zijun;LUO Li;ZHAO Junyan;ZHAO Wenbo(Department of Orthopaedics,Section One,The Fifth Affiliated(Zhuhai)Hospital of Zunyi Medical University,Zhuhai 519110,Guangdong,China)

机构地区：[1]遵义医科大学第五附属(珠海)医院骨一科,广东珠海519110

出　　处：《系统医学》2024年第15期5-9,共5页Systems Medicine

基　　金：2021年度珠海市社会发展领域科技计划医疗卫生项目(ZH22036201210088PWC)。

摘　　要：目的 研讨不同负压封闭引流压力与大鼠创面中厚皮片移植疗效的关系。方法 目的选取2022年8月—2023年10月遵义医科大学第五附属(珠海)医院实验室购买的48只实验大鼠,均行创面中厚皮片移植术,根据负压值大小及治疗方案分为低负压组(负压值75 mmHg)、中负压组(负压值125 mmHg)、高负压组(负压值175 mmHg)及打包加压组,比较组间移植疗效。结果 术后7、14 d中负压组皮片存活率均高于低负压组、高负压组和打包加压组,差异有统计学意义(P均<0.05)。中负压组并发症总发生率0,低于低负压组、高负压组、打包加压组的16.70%(2/12)、33.30%(4/12)、50.00%(6/12),差异有统计学意义(χ^(2)=8.889,P<0.05)。术后7、14 d中负压组微血管密度水平均高于低负压组、高负压组和打包加压组,差异有统计学意义(P均<0.05)。术后7、14 d中负压组血管内皮生长因子、碱性成纤维细胞生长因子蛋白表达水平均高于低负压组、高负压组和打包加压组,差异有统计学意义(P均<0.05)。结论 大鼠创面中厚皮片移植最佳的负压封闭引流压力为125 mmHg,能提高皮片存活率,促进VEGF、bFGF等生长因子表达,降低并发症发生率。Objective To investigate the relationship between different negative pressure sealing drainage pressure and the effect of medium and thick skin graft on rat wounds.Methods A total of 48 experimental rats were purchased from the laboratory of The Fifth Affiliated(Zhuhai)Hospital of Zunyi Medical University from August 2022 to October 2023 were objectively selected to undergo medium thick skin transplantation.According to the negative pressure value and treatment plan,they were divided into low negative pressure group(negative pressure value 75 mmHg),medium negative pressure group(negative pressure value 125 mmHg),high negative pressure group(negative pressure value 175 mmHg)and packing pressure group,and the transplantation efficacy of tifferent groups was compared.Results The survival rate of skin in medium negative pressure group was higher than that in low negative pressure group,high negative pressure group and packing pressure group at 7 and 14 days after operation,the differences were statistically significant(all P<0.05).The total complication rate of medium negative pressure group was 0,which was lower than that of low negative pressure group,high negative pressure group and packing pressure group 16.70%(2/12),33.30%(4/12)and 50.00%(6/12),the differences were statistically significant(χ^(2)=8.889,P<0.05).The microvascular density level of medium negative pressure group was higher than that of low negative pressure group,high negative pressure group and packing pressure group at 7 and 14 days after operation,the differences were statistically significant(all P<0.05).The protein expression levels of vascular endothelial growth factor and basic fibroblast growth factor in medium negative pressure group were higher than those in low negative pressure group,high negative pressure group and pack-ing pressure group at 7 and 14 days after surgery,the differences were statistically significant(all P<0.05).Conclu-sion The optimal negative pressure sealing drainage pressure is 125 mmHg,which can improve the survival

关 键 词：中厚皮片移植 创面 负压封闭引流 不同负压 大鼠 

分 类 号：R4[医药卫生—临床医学]